Biomolecular Analysis Service Line
Mayo Clinic's Biomolecular Analysis Service Line comprises high-complexity cores offering top-of-the-line biomolecular research support. Cores are focused on genome analysis, proteomics, metabolomics, immunochemistry and cytogenetics.
Specialized services include gene sequencing, gene analysis and genotyping; protein identification, quantification and purification; automated chemistry analysis, immunoassay analysis and small molecule analysis; cell culture, fluorescence in situ hybridization (FISH), and production of novel FISH probes; and many more.
Genome Analysis Core
The Genome Analysis Core offers researchers a synthesis of three primary services — sequencing, gene expression and genotyping — that together provide comprehensive genomic analyses. Services provide clinicians and researchers with comprehensive information about differences and changes in an individual's genetic makeup, advancing genetic and genomic understanding of many diseases and treatments.
Read more about the Genome Analysis Core.
The Proteomics Core provides proteomics and proteomics-related services, including:
- Protein identification
- Label-free differential proteomics
- Protein profiling
- Identification of post-translational modification (PTM) sites
- Targeted protein quantification
- Solid-phase peptide synthesis
- Protein purification
The core also provides services to prepare samples for proteomics analysis, including:
- Gel electrophoresis
- Protein extraction from tissues and cell cultures
- Laser capture microdissection of frozen and paraffin-embedded tissue
Read more about the Proteomics Core.
The Immunochemical Core is a specialized facility that provides immunochemical, chemistry and immune testing at an affordable cost to clinical and basic science researchers, develops and validates new assays, and improves current assay methodology. Services include:
- Automated chemistry analysis
- Immunoassay analysis
- Small molecule analysis
- Enzyme-linked immunosorbent (ELISA), Meso Scale Discovery (MSD), and radioimmunoassay (RIA) procedures for various analytes
Read more about the Immunochemical Core.
The Cytogenetics Core analyzes human and animal model research samples using methods such as:
- Cell culture
- Routine cytogenetic analysis
- Interphase and metaphase fluorescence in situ hybridization (FISH)
- Homebrew FISH probe production of custom, novel FISH probes
The core also provides training on molecular cytogenetic techniques, equipment, setup and analysis so that researchers can use the core's facilities to process and analyze their own specimens.
Read more about the Cytogenetics Core.
RNA Interference Core
The RNA Interference Core provides support for Mayo Clinic investigators who wish to knock down expression of specific mRNAs in cell lines. Services include:
- Whole-transcriptome coverage from the Sigma-Aldrich Mission library of small hairpin RNA (shRNA) lentiviral constructs
- Access to the Open Biosystems shRNA lentiviral library maintained at the University of Minnesota
This core is located at Mayo's campus in Jacksonville, Florida.
Read more about the RNA Interference Core.
The Metabolomics Core offers mass spectrometry techniques to support research in disease diagnosis and prevention by assisting investigators with profiling the metabolites involved in micro (cellular) and macro (organ) physiology. The core offers stable isotope-labeled metabolic studies in both human and animal models. Services include:
- Three complementary techniques for untargeted metabolomics profiling
- Targeted panels for mass spectrometry quantification
- Isotope-labeled ratio measurements
- Access services
The core offers a full-service facility with applications for high-field nuclear magnetic resonance (NMR) spectroscopy and biochemical and biological systems microscopy. The facility contains high-field, state-of-the-art NMR spectrometers for data acquisition, computers for NMR data analysis and a fully equipped wet laboratory for sample preparation.
Read more about the Metabolomics Core.