Gene Analysis Shared Resource

The Gene Analysis Shared Resource combines the Genome Analysis Core of the Medical Genome Facility within the Mayo Clinic Center for Individualized Medicine and the Mayo Clinic RNA Interference Core to provide Mayo Clinic Cancer Center investigators with services related to DNA and RNA sequencing, gene expression profiling, genotyping, and RNA interference.

Both the Genome Analysis Core and the RNA Interference Core have specialized scientific expertise. By bringing these two cores together as a unified, coordinated shared resource, Mayo Clinic Cancer Center researchers are given a single starting point for cancer research studies involving genomics and gene analysis.

Eric D. Wieben, Ph.D., of Mayo Clinic in Rochester, Minnesota, is the director of the Gene Analysis Shared Resource.

Genome Analysis Core

The Genome Analysis Core provides detailed information about differences and changes in an individual's genetic makeup, helping clinicians and researchers better understand the role that genetics plays in diseases and their treatments.

Co-directors of the Genome Analysis Core are Eric D. Wieben, Ph.D., Julie M. Cunningham, Ph.D., and Jin Jen M.D., Ph.D., all of Mayo Clinic in Rochester, Minnesota.

The Genome Analysis Core offers three types of services:

  • Sequencing
  • Gene expression
  • Genotyping

Sequencing services

The Genome Analysis Core provides a full range of DNA and RNA sequencing services, including Sanger Sequencing and short- and long-read next-generation sequencing.

It also offers many services and applications for next-generation sequencing, including:

  • Library preparation services
  • Whole-exome capture and custom capture
  • Whole-genome sequencing
  • Whole-transcriptome sequencing
  • Mate-pair sequencing
  • Reduced-representation bisulfite sequencing
  • MicroRNA sequencing
  • Single-cell transcriptome sequencing

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Gene expression services

The Genome Analysis Core provides comprehensive services and technical support for the measurement of gene expression, including:

  • Gene expression assays using Affymetrix GeneChip, Illumina BeadChip, Fluidigm Dynamic Array and Applied Biosystems
  • Single-cell gene expression analysis using Fluidigm C1 technologies

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Genotyping services

The Genome Analysis Core provides comprehensive genotyping and methylation analysis services on a variety of platforms, including Agena/Sequenom, Illumina, Infinium, Qiagen, pyrosequencing, Fluidigm Biomark, Agilent Array CGH and Applied Biosystems.

The Genome Analysis Core provides:

  • Whole-genome genotyping
  • Mid- to high-plex genotyping
  • Copy-number variation (CNV) analysis
  • Methylation analysis
  • Single nucleotide polymorphism (SNP) arrays

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RNA Interference Core

The RNA Interference Core offers services related to RNA interference (RNAi), a cellular mechanism that involves the silencing of gene expression.

Using RNA interference strategies, Mayo Clinic Cancer Center researchers can investigate the effects of knocking down particular genes that play roles in cancer, which may ultimately lead to new cancer treatment options.

E. Aubrey Thompson, Ph.D., of Mayo Clinic in Jacksonville, Florida, is the director of the RNA Interference Core.

The core provides investigators with:

  • Short hairpin RNA (shRNA) knockdown reagents, including the Mission library from Sigma-Aldrich and the GIPZ library from Thermo Scientific
  • Inducible shRNA knockdown reagents from Sigma-Aldrich, which allow for more precise control in regulating gene expression

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Watch a video about the Gene Analysis Shared Resource.