CryoEM support and training

The Structural Biology Core has state-of-the-art equipment and experienced staff who can prepare cryo-electron microscopy (cryoEM) grids for researchers.

CryoEM is a technique used to visualize biological molecules at near-atomic resolution without requiring staining or crystallization. In this method, purified samples are applied to a special grid. These samples are rapidly frozen in a thin layer of vitreous ice. Then they are examined with an electron microscope to capture thousands to millions of 2D projection images of individual particles in random orientations. These images are computationally combined to reconstruct 3D models of the molecules.

CryoEM is particularly useful for studying the structure of large macromolecular assemblies in their native, hydrated state, including proteins and viruses, providing insights into how their molecular structures relate to their function.

Training available

Training is available for grid preparation, grid screening, data collection and data processing.

For consultation or questions regarding structural biology projects and services, submit your request with this intake form (must be logged into the Mayo Clinic network).