Self-service equipment

The Multiomics Mass Spectrometry Core provides access to cutting-edge specialty instruments for proteomics sample preparation through a self-service, fee-for-use model. This equipment supports applications in single-cell, spatial and biofluid proteomics, as well as other low-input workflows.

The core manages scheduling and billing. The core does not perform operations on users' behalf, but core-operated services for single-cell omics are being developed. Prior training is required for independent access.

For access and training information, contact the Multiomics Mass Spectrometry Core.

CellenONE X1: Automated single-cell isolation and dispensing

The CellenONE X1 combines imaging-based single-cell detection with microfluidic dispensing to enable gentle, accurate isolation of single cells in nanoliter volumes for downstream single-cell omics applications.

Applications

• Single-cell omics, including proteomics, transcriptomics and genomics.
• Rare cell isolation and enrichment.
• Nanoliter-scale reagent dispensing.
• High-throughput screening and miniaturized assays.
• Sample processing of microdissected tissue.

Access and training

For access and training, email the core.

KingFisher Apex: Automated magnetic bead purification

The KingFisher Apex automates high-throughput magnetic bead-based purification of proteins, nucleic acids and cells. It supports diverse proteomics workflows, including immunoprecipitation, affinity-purification of biotinylated proteins with streptavidin-beads and enrichment of low-abundance proteins from biofluids.

Applications

• Antibody bead-based affinity purification (immunoprecipitation-mass spectrometry).
• Isolation of proximity-biotinylated proteins from cells or tissues (BioID and APEX).
• Click chemistry-based enrichment strategies, such as bio-orthogonal noncanonical amino acid tagging (BONCAT).
• Magnetic nanoparticle protein corona enrichment from biofluids.
• High-throughput sample cleanup and enrichment.

Access and training

For access and training, email the core.

Leica LMD7: Laser capture microdissection system

The Leica LMD7 enables contact-free, contamination-free isolation of defined regions, cells or structures from frozen or formalin-fixed paraffin-embedded tissue sections for downstream molecular analysis, including proteomics.

Applications

• Spatial transcriptomics and proteomics.
• Deep visual proteomics.
• Isolation of specific cell types.
• Single-cell proteomics.

Access and training

For access and training, email the core.

Syncell Microscoop: Image-guided photolabeling for spatial proteomics

The Syncell Microscoop enables image-guided, high-precision photobiotinylation of proteins within subcellular regions of interest in cultured cells or tissue sections. Labeled proteins can be affinity-purified and analyzed by liquid chromatography-tandem mass spectrometry to reveal spatially resolved proteomes.

Application

• Spatial discovery proteomics in cells and tissues at subcellular resolution.

Access and training

For access and training, email the core.

Consultation services

If you are a new user of the core, a consultation with our research scientist is required before the core can accept samples for your proteomics project. This consultation ensures that the core offers you the highest-quality services.

The core reserves two hours weekly for no-charge project consultations.

For consultations or more information on access, training and service fees, email the core.