About the Lab

The Regenerative Physiology Laboratory of Carlos B. Mantilla, M.D., Ph.D., at Mayo Clinic studies the control of breathing. The lab's long-term regenerative physiology goal is to develop rational and effective therapies for the treatment of diseases that impair the ability to breathe independently.

The Regenerative Physiology Lab makes extensive use of cutting-edge and state-of-the-art physiological, neuroscience and biomedical engineering techniques to evaluate the function of motor neurons and the muscle fibers they innervate. These tools include:

  • High-fidelity electrophysiological recordings and measurements of single and multiple motor unit activity
  • Laser capture microdissection and quantitative transcriptional analyses in single motor neurons
  • Multicolor, 3-D confocal imaging
  • Targeted experimental and therapeutic manipulations of gene expression in motor neurons and muscle fibers
  • Regenerative medicine techniques for cell delivery and engineering
  • Nanoparticle synthesis and engineering with targeted diagnostic and therapeutic potential

The lab uses whole-body plethysmography, lung mechanics and wireless telemetry of respiratory function to assess the impact of physiological changes in motor neurons, muscle fibers and neuromuscular junctions across diseases and conditions that limit the ability to sustain breathing and perform expulsive maneuvers such as coughing and sneezing that are necessary to maintain clear airways.