This image shows representative diaphragm muscle cross-sections with type I fibers in blue, type IIa fibers in purple, and type IIx or type IIb fibers both in black. Scale bar is 50 μm. Individual muscle fibers are automatically thresholded and separated for analysis. Greising SM, et al. Analysis of muscle fiber clustering in the diaphragm muscle of sarcopenic mice. Muscle & Nerve. 2015;52:76.