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Laboratories

Lung Development and Regeneration: Douglas G. Brownfield

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  • Confocal microscopy mapping construction of mouse lung

    Mapping construction of the lung by confocal microscopy

    Immunostain of an adult mouse lung reveals precise microenvironmental patterning.

  • Single-cell RNA-seq (scRNA-seq) used to reconstruct cell fate dynamics in mouse lung cells

    Reconstructing cell fate dynamics via single-cell RNA-seq (scRNA-seq)

    Uniform Manifold Approximation and Projection (UMAP) plot integrating Velocity with Leiden analysis of mouse lung cells from late embryonic timepoints.

  • Green fluorescent protein (GFP)-labeling reveals fate plasticity in genetically engineered mouse models.

    Fate plasticity revealed through use of genetically engineered mouse models

    Lineage tracing via green fluorescent protein (GFP)-labeling shows fate conversion upon deletion of an identified transcription factor.

Dr. Brownfield's Lung Development and Regeneration Lab studies lung development, repair and regeneration with the goal of improving the treatment of pulmonary disease. Using single-cell transcriptomics, genetically engineered mouse models and three-dimensional organotypic culture, Dr. Brownfield's lab maps the lung's cellular and molecular components at single-cell resolution over time and identifies regulators of its proper construction.

The lab's multidisciplinary team applies their findings in adults to determine whether regulators are reinitiated after injury, disrupted in disease, or useful in the development of regenerative therapies that repair or replace defective tissue.

About Dr. Brownfield

Douglas G. Brownfield, Ph.D., is a senior associate consultant in the Division of Pulmonary and Critical Care Medicine at Mayo Clinic in Rochester, Minnesota. Previously, he was the Mark and Catherine Winkler Assistant Professor of Cell and Developmental Biology in the Department of Environmental Health at Harvard University.

Dr. Brownfield earned a Bachelor of Science degree in biomedical engineering from Tulane University followed by a doctorate degree in bioengineering from the University of California, Berkeley, where he was mentored by Mina J. Bissell, Ph.D., and minored in management of technology through the Haas School of Business. He completed a postdoctoral fellowship in the Department of Biochemistry at Stanford University, while being mentored by Mark A. Krasnow, M.D., Ph.D.

Awards received by Dr. Brownfield include the National Institutes of Health Pathway to Independence Award, American Cancer Society Postdoctoral Fellowship and the National Science Foundation Graduate Research Fellowship Program.

  • See Dr. Brownfield's full faculty bio for more information about his professional highlights.
  • Review Dr. Brownfield's published research.

Training opportunities

Learn about opportunities available to trainees in Dr. Brownfield's Lung Development and Regeneration Lab at Mayo Clinic.
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