Targeted Metabolomics

The Mayo Clinic Metabolomics Core has established a large number of targeted panels for quantitation of selected groups of metabolites using mass spectrometry. The key distinction between untargeted and targeted measurements is that these targeted panels provide accurate absolute quantitation of a focused group of analytes using available standards.

Targeted metabolomics is a quantitative approach in which a known set of metabolites is quantitated using 13C, 2H or 15N isotope-labeled internal or external reference compounds. The resulting data can then be used as input variables for statistical analysis.

The Metabolomics Core's panels include many metabolites of intermediary metabolism, lipids, carboxylic acids, organic acids, neuromodulators and drug metabolites. These types of panels are established to meet the needs of investigators on a case-by-case basis. Investigators who are interested in measuring metabolites not listed below should contact the core director to discuss possibilities for establishing a method.

Amino acids (AA) panel: 42 analytes

Histidine Hydroxyproline 1-methylhistidine 3-methylhistidine
Asparagine Phosphoethanolamine Arginine Carnosine
Taurine Anserine Serine Glutamine
Ethanolamine Glycine Aspartic acid Sarcosine
Citrulline Glutamic acid Beta-alanine Threonine
Alanine Gamma-amino-n-butyric acid Alpha-aminoadipic acid Beta-aminoisobutyric acid
Proline Hydroxylysine 1 Hydroxylysine 2 Alpha-amino-n-butyric acid
Ornithine Cystathionine 1 Cystathionine 2 Lysine
Cysteine Tyrosine Methionine Valine
Isoleucine Allo-isoleucine Homocysteine Leucine
Phenylalanine Tryptophan

Neuromodulators (NM) panel: 13 analytes

Acetylcholine Adenosine Histidine Serine
Taurine Glutamine Glycine Aspartic acid
Glutamic acid Gamma-amino-n-butyric acid Norepinephrine Dopamine

Tricarboxylic acids (TCA) panel: 12 analytes

Lactic acid Fumaric acid Succinic acid Oxaloacetic acid
Alpha-ketoglutaric acid Malic acid Cis aconitic acid Citric acid
Isocitric acid Glutamic acid 2-hydroxyglutarate Aspartate

Nonesterified fatty acids (NEFA) panel: 12 analytes

Myristic acid Palmitic acid Palmitoleic acid Palmitelaidic acid
Stearic acid Oleic acid Elaidic acid Linoleic acid
Alpha-linolenic acid Arachidonic acid Eicosapentaenoic acid Docosahexaenoic acid

Short-chain fatty acids (SCFA) panel: 8 analytes

Acetic acid Propionic acid Isobutyric acid Butyric acid
Valeric acid Isovaleric acid Isocaproic acid Hexanoic acid

Sphingolipids panel: 12 analytes

Sphingosine Sphinganine Sphingosine-1-phosphate 18C8-ceramide
18C14-ceramide 18C16-ceramide 18C18:1-ceramide 18C18-ceramide
18C20-ceramide 18C22-ceramide 18C24:1-ceramide 18C24-ceramide

Hexosylceramides panel: 14 analytes

Galactosyl(beta) sphingosine C12-galactosyl(beta) ceramide C16-galactosyl(beta) ceramide C18-galactosyl(beta) ceramide
C18:1-galactosyl(beta) ceramide C24-galactosyl(beta) ceramide C24:1-galactosyl(beta) ceramide Glucosyl(beta) sphingosine
C12-glucosyl(beta) sphingosine C16-glucosyl(beta) sphingosine C18-glucosyl(beta) sphingosine C18:1-glucosyl(beta) sphingosine
C24-glucosyl(beta) sphingosine C24:1-glucosyl(beta) sphingosine

Diacylglycerols (DAG) panel: 13 analytes

1,2-12:0/12:0 DAG 1,2-14:0/14:0 DAG 1,2-16:0/16:0 DAG 1,2-16:0/18:2 DAG
1,2-16:0/18:1 DAG 1,2-18:0/16:0 DAG 1,2-18:1/18:2 DAG 1,2-18:1/18:1 DAG
1,2-18:0/18:2 DAG 1,2-18:0/18:0 DAG 1,3-18:2/18:2 DAG 1,2-18:0/20:4 DAG
1,2-18:0/22:6 DAG

Acylcarnitines panel: 14 analytes

Carnitine Acetylcarnitine Propionylcarnitine Isobutyrylcarnitine
Butyrylcarnitine Isovalerylcarnitine Octanoylcarnitine Decanoylcarnitine
Lauroylcarnitine Myristoylcarnitine Palmitoylcarnitine Linoleoylcarnitine
Oleoylcarnitine Stearoylcarnitine

Trimethylamine (TMAO) panel: 5 analytes

Carnitine Trimethylamine Trimethylamine n-oxide Choline

Bile acids panel: 18 analytes

Cholic acid Chenodeoxycholic acid Deoxycholic acid Glycocholic acid
Glycochenodeoxycholic acid Glycodeoxycholic acid Glycolithocholic acid Glycoursodeoxycholic acid
Hyodeoxycholic acid Lithocholic acid Alpha+omega-muricholic acid Beta-muricholic acid
Taurocholic acid Taurochenodeoxycholic acid Taurodeoxycholic acid Taurolithocholic acid
Tauroursodeoxycholic acid Ursodeoxycholic acid

Endocannabinoids panel: 4 analytes

2-arachidonoyl glycerol Arachidonoylethanolamide
Oleoylethanolamide Palmitoylethanolamide

Alpha-hydroxyglutaric acid isomers: 2 analytes

D-alpha-hydroxyglutaric acid L-alpha-hydroxyglutaric acid

Ketoacids panel: 3 analytes

Alpha-ketoisocaproic acid Alpha-keto-beta-methylvaleric acid Alpha-ketoisovaleric acid

Individual drug target

Metformin Acamprosate Valproic acid Nicardipine
Nimodipine Acesulfame potassium Tryptamine

The Metabolomics Core also has a Bruker IVDr platform for automation analysis of human plasma and urine samples. Visit the Bruker website to learn more.