Facilities and Equipment

Facilities

The Metabolomics Core facilities are divided into two locations at Mayo Clinic in Rochester, Minnesota:

  • Mass spectrometry facilities. Mass spectrometry facilities occupy approximately 3,500 square feet in the Alfred Building at Mayo Clinic Hospital — Saint Marys Campus. The facilities comprise two instrument rooms, a work-up laboratory and office space, and a conference room.
  • Nuclear magnetic resonance (NMR) facilities. NMR spectroscopy facilities include approximately 3,100 square feet in the basement of the Stabile building, housing instruments, offices and a wet lab.

Equipment

Mass spectrometry equipment

The Mayo Clinic Metabolomics Core has 14 mass spectrometers divided into two categories: single quadrupole and tandem instruments.

Single quadrupole instruments include:

  • Agilent Technologies 5973N MSD
  • Agilent Technologies 5975C MSD
  • Agilent Technologies 5977A MSD
  • Agilent Technologies 5977B MSD

Tandem instruments include:

  • Applied Biosystems Sciex 6500 (LC/MS/MS)
  • Agilent Technologies 6460 (LC/MS/MS)
  • Agilent Technologies 6550iFunnel Q-TOF (LC/MS/MS) x 2
  • ThermoFisher Scientific TSQ Quantum Ultra (LC/MS/MS) x 3
  • ThermoFisher Scientific TSQ Quantiva (LC/MS/MS) x 2
  • ThermoFisher Scientific Q-Exactive Plus (LC/MS/MS)

NMR spectroscopy equipment

The Mayo Clinic Metabolomics Core's NMR equipment includes:

  • Bruker DRX-700 (700 MHz 1H, 16.4 tesla) 3-channel equipped with cryoprobe (5 mm triple resonance 1H, 13C, 15N, proton detection, and single axis gradient), 5 mm broadband (15N to 31P, 51 to 209 MHz) with 1H decoupling for proton and heteronuclear spectroscopy.
  • Bruker Avance III 600 MHz spectrometer, a high-resolution 1H NMR, with high-throughput sample handler for metabolomics studies.
  • Bruker 500 MHz spectrometer (Avance) used for 18O-assisted 31P NMR studies of cellular phosphoryl-containing metabolites.
  • Two NMR spectrometers (the 300 and 700 MHz wide bore) are equipped with mini- and microimaging accessories that enable recording of NMR images and high-resolution NMR spectra of small animals, perfused organs, tissues and cell cultures. The 300 MHz spectrometer has a magic angle spinning probe for high-resolution multinuclear spectroscopy of soft solids such as tissue, cell pellets and the like.
  • The NMR spectroscopy facility is equipped with organ perfusion systems, cell culture and whole-blood metabolic labeling tools. It has Hewlett Packard HPLC HP1100 for fractionation, purification and quantification of metabolites and their respective phosphoryls.