Reprogramming

Reprogramming services that the core provides include:

• Induced pluripotent stem cell production by CytoTune-iPS 2.0 Sendai virus reprogramming of fibroblasts and peripheral blood mononuclear cells. The core uses the CytoTune-iPS 2.0 Sendai virus-based reprogramming system to efficiently generate human induced pluripotent stem cells from dermal fibroblasts and peripheral blood mononuclear cells. This method ensures a robust reprogramming process that maintains the pluripotency of the cells, offering a reliable source of induced pluripotent stem cells for a variety of research applications.
• Skin fibroblasts derived from dermal skin punch for isolation, expansion, cryopreservation and reprogramming into induced pluripotent stem cells. Skin fibroblasts are isolated from a dermal skin punch biopsy, a minimally invasive procedure that provides a rich source of cells. Then these fibroblasts are expanded in culture, cryopreserved for long-term storage and subsequently reprogrammed into induced pluripotent stem cells. This process ensures that a diverse collection of cell lines is available for reprogramming into induced pluripotent stem cells, providing researchers with flexible and scalable options.
• Plans to build a bank of reprogrammed induced pluripotent stem cells for client access. The core is working to establish a comprehensive biobank of reprogrammed induced pluripotent stem cells, allowing researchers to easily access already existing aliquots for their own studies. This biobank will be a valuable resource for clients, reducing the time and effort required to generate new induced pluripotent stem cells and supporting a wide range of research initiatives. Clients will be able to request specific cell lines or access a broad selection of preexisting induced pluripotent stem cells for various experimental needs.

For more information, contact the core.