CRISPR single guide RNA cloning

Single guide RNA (sgRNA) constructs are essential for screening hits validation and mechanistic studies. The Genetic Screening and Engineering Core can assist with the design and cloning of sgRNAs for targeted gene engineering, including:

• CRISPR knockout (CRISPR-KO).
• CRISPR interference (CRISPRi).
• CRISPR activation (CRISPRa).
• Sequence editing.

For questions about core services, contact the core.