Overview
The Genetic Screening and Engineering Core specializes in high-throughput functional genetic screening and cellular engineering. The core offers state-of-the-art tools to precisely modify the genome of cells and organisms using CRISPR-Cas9-based editing, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) systems. Our platforms enable high-throughput genetic screening, gene knockouts, transcriptional modulation, and sequence editing with high efficiency and scalability.
Mayo Clinic's Genetic Screening and Engineering Core collaborates with investigators to fully support CRISPR-based projects, including library design, construction, screening, sequencing and data analysis. The core also offers technical expertise for gene editing and cellular engineering projects.
We support projects ranging from single-gene edits to genome-wide pooled screens, all tailored for your research needs. These tools are indispensable across a wide range of research areas, including:
- Cancer biology. CRISPR-based approaches help investigate tumor suppressors, oncogenes and drug resistance mechanisms.
- Cell therapy. Precision gene editing underpins the development of engineered T cells and stem cell models.
- Functional genomics. CRISPR screens provide powerful tools for elucidating novel functional genes and their networks. Our featured CRISPR gene tiling approach further pushes the boundary by mapping protein domain function and advancing therapeutic development with unmatched resolution.
The Genetic Screening and Engineering Core aims to play a critical role in translational research at Mayo Clinic by serving as a bridge between bench science and clinical innovation. Our technologies support the creation of accurate disease models and the validation of therapeutic targets and preclinical testing pipelines. By integrating with other clinical research cores, the core empowers investigators to translate genetic discoveries into novel diagnostics and therapeutics.
For questions about core services, contact the core.