Techniques

The Mayo Clinic Brain Tumor Patient-Derived Xenograft National Resource provides information about techniques for establishing brain tumor PDX lines for research. Here, we explain our methodology for establishing PDX models, serial passaging, in vitro culture and more.

  • Establishing a PDX. The methodology used to establish PDX models in the PDX National Resource involves removing patient tumor tissue during surgery and transporting it in DMEM medium to the laboratory. Read about establishing a PDX.
  • Serial passaging. The PDX National Resource follows a specific methodology for serial passaging of patient-derived xenograft models as subcutaneous tumors. Read about serial passaging.
  • In vitro culture. Short-term explant cultures derived from tumor xenograft tissue in the PDX National Resource can be used for in vitro cell culture-based assays. Read about in vitro cultures.
  • Intracranial tumor implantation. The PDX National Resource follows a specific methodology for injecting a cell suspension into the brain. Read about intracranial tumor implantation.
  • Flank tumor implantation. The PDX National Resource follows a specific methodology for flank tumor implantation. Read about flank tumor implantation.
  • Cryopreservation. The PDX National Resource follows a specific methodology for cryopreservation of flank tumors and restoration of flank tumors. Read about cryopreservation.