Establishing a PDX

The methodology used to establish patient-derived xenograft models in the Mayo Clinic Brain Tumor Patient-Derived Xenograft National Resource involves removing tumor tissue from a patient during surgery and transporting it in DMEM medium to the laboratory.

The tumor tissue is mechanically disaggregated, triturated through a 1 milliliter-syringe and mixed with reduced growth factor Matrigel in a 1:1 ratio. Up to 200 microliters of this mixture is then injected subcutaneously in the flank of nude mice.

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