Extracellular Vesicles for Tissue Regeneration

Extracellular vesicles are biological nanoparticles that contain biomolecules, such as RNAs and proteins.

Dr. Wolfram's laboratory is developing biological nanoparticles for liver repair.

Extracellular vesicles (EVs) are biological nanoparticles secreted by all cells. Extracellular vesicles contain biomolecules, such as RNA, proteins, lipids and carbohydrates, which play an important role in cell communication.

Recent evidence suggests that the beneficial effects of stem cells in tissue regeneration are largely due to paracrine effects mediated by extracellular vesicles.

Dr. Wolfram's Nanomedicine and Extracellular Vesicles Lab studies the effect of extracellular vesicles on tissue regeneration and uses novel methods for extracellular vesicle isolation from cell culture media and biological fluids.

One major focus of Dr. Wolfram's research team is using extracellular vesicles to improve liver regeneration and transplantation. The liver is an ideal target for extracellular vesicle therapeutics because more than 90 percent of systemically administered nanovesicles accumulate in the liver.

The Mayo Clinic EV-Therapeutics Program supports Dr. Wolfram's research efforts. The focus of the EV-Therapeutics Program is to develop scalable manufacturing processes for bringing stem cell-derived extracellular vesicles to clinical practice.