Devices for Point-of-Care Testing
Figure 4A. A multichamber microfluidic device for blood analysis. Each chamber may contain a different leukocyte subset or be perfused with a different stimulant.
Figure 4B. A concept of antibody arrays used for capturing cells and detection of secreted cytokines.
Figure 4C. T cells captured next to antibody spots detecting IFN-γ and IL-2 secreted by this group of cells.
Another long-standing interest in the lab is aimed at capturing leukocytes and detecting secreted cytokines to attain diagnostic information. In a series of publications, researchers demonstrated that antibody microarrays can be combined with microfluidic devices to create a platform where specific leukocyte subsets are captured in close proximity to antibody spots specific for cytokines of interest.
The same device (see Figure 4C) may be used to capture and purify leukocyte subsets of interest from complex biological fluid such as blood, and then carry out cytokine profiling for the same subset. Importantly, such devices require only a small volume of sample and may be suited for small animal research, pediatric immunology or other applications where sample volume is limited.
Zhu H, Stybayeva G, Macal M, Ramanculov E, Dandekar S, George MD, Revzin A. A microdevice for multiplexed detection of T-cell-secreted cytokines. Lab on a Chip. 2008;8:2197.
Stybayeva G, Mudanyali O, Seo S, Silangcruz J, Macal M, Ramanculov E, Dandekar S, Erlinger A, Ozcan A, Revzin A. Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function. Analytical Chemistry. 2010;82:3736.
Vu T, Rahimian A, Stybayeva G, Gao Y, Kwa T, Van de Water J, Revzin A. Reconfigurable microfluidic device with integrated antibody arrays for capture, multiplexed stimulation, and cytokine profiling of human monocytes. Biomicrofluidics. 2015;9:044115.