My research interests are in three areas:
Please note that each of these projects involves an ongoing collaboration with Dr. Keith A. Jones and Dr. David O. Warner. The PI for each project is mentioned in brackets at the end of the description.
The mechanisms by which chronic NO treatment reduces NO responsiveness in vascular smooth muscle This work examines the effects of chronic NO treatment on the activity and isoform and subtype expression of the primary enzymes that mediate smooth muscle responsiveness to NO, specifically soluble guanylate cyclase (a heterodimer)and cGMP-dependent protein kinase. The mechanisms by which NO exerts these effects on these enzymes is also being investigated. This work primarily focuses on a cultured pulmonary artery preparation at this time. (WJP)
The mechanisms by which oxidants, such as nitric oxide, peroxynitrite, and hydrogen peroxide reduce the amount of force at a given myoplasmic calcium This work has initially characterized classes of oxidant sensitive targets and two primary protein targets have been identified, myosin light chain kinase and the smooth muscle myosin head. Ongoing work will characterize the chemical nature of the oxidation mediated reduction in enzyme activity in these proteins that are critical for normal enzyme function. (KAJ)
The mechanisms by which volatile anesthetic agents and alcohols decrease agonist-induced calcium sensitization in airway smooth muscle This work has focused on characterizing the specific signaling pathways through which anesthetics and alcohols mediate the aforementioned effects. The current focus is on anesthetic effects on receptor-G-protein coupling, heterotrimeric G-protein activity and downstream effectors present in airway smooth muscle. (DOW/KAJ)