Sanger sequencing

Mayo Clinic Molecular Biology Core is partnering with GENEWIZ for Sanger Sequencing. This partnership provides:

  • Next business day turnaround time
  • Expert technical support
  • Convenience, including on-site drop boxes and free sample shipping
  • Preferred pricing

To order, create a GENEWIZ account or select Register on the GENEWIZ home page.

During registration, use PI/Institution ID# 315588 for new user accounts. Existing users may use the existing PI/Institution ID assigned to other researchers in the lab.

For the billing address, use:

  • Mayo Foundation
  • Accounts Payable, Dept. 10000
    200 First St. SW
    Rochester, MN 55905

For optimal sequencing results, please prepare samples according to the Sample Submission Guidelines. The results will be available the following business day.

GENEWIZ drop box locations:

  • Molecular Biology Core, Stabile Building, Room 13-21
  • Guggenheim Building, Room 16-18
  • Gonda Building, 19th Floor, Room 306
  • Mayo Clinic Florida – Griffin Building, Room 341A, 3 p.m. (notify lab member)
  • Mayo Clinic Scottsdale, Arizona – TBD

Note: Cut-off time for samples is 4 p.m. for all Rochester drop boxes.

To request additional drop boxes or for pricing information, contact:

Michael Bullard

Next-generation sequencing

Next-generation sequencing is the process of sequencing DNA or RNA by producing millions of sequence reads in a massively parallel manner. Also known as deep sequencing, next-generation sequencing allows for the sequencing of the entire genome, exome or transcriptome. This makes it possible to identify complex genetic differences that may cause disease or help diagnose and treat disease.

  • Consultations. A free-of-charge consultation is required before submitting a service request for next-generation sequencing. The consultation will include discussion of experimental design, sample requirements, submission procedures, costs and data analysis requirements.
  • Results. Next-generation sequencing produces much larger data sets than does Sanger sequencing. Results are delivered as FASTQ or BAM files to the next-generation sequencing data delivery server. While investigators can perform their own downstream analysis, they are encouraged to work with the Mayo Clinic Bioinformatics Core due to the size and complexity of the data. The Molecular Biology Core and Bioinformatics Core have integrated work flows to take advantage of the various analytical pipelines developed by the Bioinformatics Core.
  • Applications. Applications of next-generation sequencing include:
    • De novo sequencing. De novo sequencing is the process of sequencing unknown genomes or transcriptomes. It may require a combination of several applications, including Sanger sequencing and short- and long-read next-generation sequencing.
    • Whole-genome sequencing. Whole-genome sequencing is the process of sequencing genomes that have a known reference sequence to identify differences and changes in an individual's genomic makeup.
    • Mate pair sequencing. Mate pair sequencing involves sequencing the ends of long fragments (2 to 5 kilobases) of DNA to identify large structural rearrangements or indels.
    • Targeted resequencing. Targeted resequencing, which includes whole-exome capture, custom capture and amplicon sequencing, refers to the process of sequencing a subset of a known genome in an effort to identify single-nucleotide polymorphisms (SNPs).
    • ChIP sequencing (ChIP-seq). ChIP-seq is sequencing in combination with chromatin immunoprecipitation to identify protein-DNA interactions that can affect gene expression.
    • Methyl sequencing (methyl-seq). Methyl-seq is sequencing in combination with bisulfite conversion to identify methylation patterns of genomic regions that can affect gene expression.
    • Whole-transcriptome sequencing. Whole-transcriptome sequencing, also known as RNA sequencing (RNA-seq), is the process of sequencing expressed genes in the genome. It can also identify splice variants, gene fusions and mutations.
    • MicroRNA sequencing (miRNA-seq). MiRNA sequencing targets the various miRNAs expressed in the cell that can control RNA translation by translational repression, target degradation and gene silencing.