Personnel working within the Gene Expression Core (GEC) at Mayo Clinic have the expertise and qualifications needed to provide top-quality expression data on a variety of different platforms. With more than 100 years of combined laboratory experience, the staff is well-suited to perform high-complexity gene transcript measurements.

Investigators at Mayo Clinic are engaged in a wide variety of basic and clinical research studies. As a result, GEC staff has significant expertise working with human and animal samples of all kinds and performing gene expression analysis on a wide variety of eukaryotic species.

Areas of GEC expertise include:

  • Gene transcript measurement in archival clinical samples. Mayo Clinic has one of the largest repositories in the world for archival tissue samples, providing institutional researchers with an unsurpassed resource for studying disease. As a result, the GEC has gained significant expertise by analyzing thousands of RNA samples derived from formalin-fixed, paraffin-embedded tissues.
  • High-throughput real-time reverse transcription quantitative PCR. The GEC routinely performs real-time reverse transcription quantitative PCR using instrument platforms from Applied Biosystems and Fluidigm. The Fluidigm system currently analyzes up to 96 samples and 96 targets in a single run and performs the reactions in only 10 nanoliters of reagent.
  • Analysis of microRNA expression. The GEC has provided services for microRNA expression analysis for several years. Recently, the core has completed a study comparing the expression levels of several types of samples across multiple platforms. This work has provided the core with a cross-platform perspective that has enhanced its ability to support genomic researchers.