Regulation of Muscle Protein Phenotype in Humans with Obesity

Overview

About this study

The purpose of this study is to determine the turnover rate of motor protein isoforms (myosin heavy chain, MHC) and overall muscle protein in skeletal muscle of subjects with obesity and lean controls in the basal state, and to determine stimulation of the turnover rate of motor protein (MHC isoforms) in skeletal muscle of subjects with obesity and lean controls by plasma amino acids and with and without prior exercise.

Participation eligibility

Participant eligibility includes age, gender, type and stage of disease, and previous treatments or health concerns. Guidelines differ from study to study, and identify who can or cannot participate. There is no guarantee that every individual who qualifies and wants to participate in a trial will be enrolled. Contact the study team to discuss study eligibility and potential participation.

Inclusion Criteria:

- ability to sign informed consent form

- body mass index (BMI), 18-26 kg/m2 (lean subjects), 32-50 kg/m2 (subjects with
obesity)

Exclusion Criteria:

- prescription or over-the-counter medication

- supplements known to affect protein metabolism (i.e., amino acids, protein, omega-3
fatty acids)

- diabetes

- acute illness

- liver disease

- uncontrolled metabolic disease, including renal disease

- heart disease related to atrial fibrillation, history of syncope, limiting or unstable
angina, congestive heart failure or ECG documented abnormalities

- low hemoglobin or hematocrit

- use of anabolic steroids or corticosteroids (within 3 months)

- not classified as inactive/sedentary based on the Stanford Brief Activity Survey and
accelerometry data

- participation in a weight-loss regimen

- extreme dietary practices (i.e., vegan, vegetarian)

- smoking

- pregnancy

- gastro-intestinal surgery

- any other condition or event considered exclusionary by the PI and the study
physician.

Note: Other protocol defined Inclusion/Exclusion Criteria may apply.

Eligibility last updated 4/8/2024. Questions regarding updates should be directed to the study team contact.

Participating Mayo Clinic locations

Study statuses change often. Please contact the study team for the most up-to-date information regarding possible participation.

Mayo Clinic Location	Status	Contact
Scottsdale/Phoenix, Ariz. Mayo Clinic principal investigator Lori Roust, M.D.	Closed for enrollment	Contact information: Brooke Brown (480) 301-4735 Brown.Brooke@mayo.edu

Mayo Clinic Location

Status

Contact

Scottsdale/Phoenix, Ariz.

Mayo Clinic principal investigator

Lori Roust, M.D.

Closed for enrollment

Contact information:

Brooke Brown

(480) 301-4735

Brown.Brooke@mayo.edu

More information

Publications

Publications are currently not available

Clinical Trials