Regulation of Muscle Protein Phenotype in Humans with Obesity

Overview

About this study

The purpose of this study is to determine the turnover rate of motor protein isoforms (myosin heavy chain, MHC)  and overall muscle protein in skeletal muscle of subjects with obesity and lean controls in the basal state, and to determine stimulation of the turnover rate of motor protein (MHC isoforms) in skeletal muscle of subjects with obesity and lean controls by plasma amino acids and with and without prior exercise. 

 

 

Participation eligibility

Participant eligibility includes age, gender, type and stage of disease, and previous treatments or health concerns. Guidelines differ from study to study, and identify who can or cannot participate. There is no guarantee that every individual who qualifies and wants to participate in a trial will be enrolled. Contact the study team to discuss study eligibility and potential participation.

Inclusion Criteria:

  • Age, 18-45 years old.
  • Ability to sign informed consent form.
  • Body mass index (BMI), 18.0 – 26.0 kg/m^2 (lean subjects).
  • Body mass index (BMI), 32.0-50.0 kg/m^2 (subjects with obesity).

Exclusion Criteria:

  • Evidence of diabetes.
  • Presence of acute illness.
  • History of liver disease.
  • Uncontrolled metabolic disease, including renal disease.
  • Heart disease related to atrial fibrillation, history of syncope, limiting or unstable angina, congestive heart failure or ECG documented abnormalities such as > 0.2 mV horizontal or downsloping ST-segment depression, or frequent arrhythmias (> 10 premature ventricular contractions/min).
  • Low hemoglobin or hematocrit.
  • Use of anabolic steroids or corticosteroids (within 3 months).
  • Not classified as inactive/sedentary based on accelerometry/ActivPal data (i.e., physical activity > 1.7 METs). 
  • Current participation in a weight-loss regimen.
  • Extreme dietary practices (i.e., vegan, vegetarian).
  • Smoking (including electronic cigarettes and marijuana) subjects will be included as long as they are willing to abstain for 5 days prior to screening and infusion studies.
  • Ppregnancy - women trying to become pregnant will be excluded from the study; women of child-bearingpotential will be able to participate in this study only if they have a negative pregnancy test and agree to use acceptable birth control during the course of the study [surgical sterilization, approved hormonal contraceptives (such as birth control pills, Depo-Provera), barrier methods (such as a condom or diaphragm) used with a spermicide, an intrauterine device (IUD), abstinence].
  • Gastro-intestinal surgery.
  • Any medication, intake of supplements, other condition, or event considered exclusionary by the PI and the study physician.

Eligibility last updated 1/24/22. Questions regarding updates should be directed to the study team contact.

Participating Mayo Clinic locations

Study statuses change often. Please contact the study team for the most up-to-date information regarding possible participation.

Mayo Clinic Location Status Contact

Scottsdale/Phoenix, Ariz.

Mayo Clinic principal investigator

Lori Roust, M.D.

Closed-enrolling by invitation

What is this? (?)
"Close"
Not open to everyone who meets the eligibility criteria, but only those invited to participate by the study team.

Contact information:

Brooke Brown

(480) 301-4735

Brown.Brooke@mayo.edu

More information

Publications

Publications are currently not available
.

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