Vaccine Therapy in Treating Patients With Previously Treated Stage II-III HER2-Positive Breast Cancer


About this study

The purpose of this study is to look at the safety and immune response to a vaccine used in patients previously treated for HER2 (human epidermal growth factor receptor 2) positive breast cancer.

Participation eligibility

Participant eligibility includes age, gender, type and stage of disease, and previous treatments or health concerns. Guidelines differ from study to study, and identify who can or cannot participate. There is no guarantee that every individual who qualifies and wants to participate in a trial will be enrolled. Contact the study team to discuss study eligibility and potential participation.

This study is ongoing, but not recruiting participants

Inclusion Criteria:

  • Histological confirmation of primary breast cancer, stage II or III, completely resected
    • Note: history of a local recurrence allowable if also completely resected
  • Prior diagnosis of HER-2 positive primary breast cancer using American Society of Clinical Oncologists (ASCO)/College of American Pathologists (CAP) guidelines (either by evidence of 3+ immunohistochemical staining or with in situ hybridization [FISH] amplification)
  • Completion of surgery +/- radiation at least 30 days prior to registration
  • Must have received mastectomy or lumpectomy plus radiation
  • Must have received either neoadjuvant and/or adjuvant chemotherapy for treatment of breast cancer
  • Must have received either neoadjuvant and/or adjuvant trastuzumab for treatment of breast cancer
  • All chemotherapy, trastuzumab, and/or corticosteroids must be completed at least 90 days prior to registration
    • Note: hormonal therapy and bisphosphonates may be ongoing
  • Clinically without any evidence of disease recurrence/progression (per practice guidelines for breast cancer)
  • Absolute neutrophil count (ANC) ≥ 1500/mm^3
  • Platelet count ≥ 75,000/mm^3
  • Hemoglobin ≥ 9.0 g/dL
  • Creatinine ≤ 2 x upper limit of normal (ULN)
  • Serum glutamic oxaloacetic transaminase (SGOT) (aspartate aminotransferase [AST]) ≤ 2 x ULN
  • Albumin ≥ 3 g/dL
  • Negative pregnancy test done ≤ 7 days prior to registration, for women of childbearing potential only
  • Capable of understanding the investigative nature, potential risks, and benefits of the study
  • Capable of providing valid informed consent
  • Willing to return to enrolling institution (Mayo Clinic Rochester) for all study visits (immunizations, blood draws, etc)
  • Willing to employ adequate contraception from the time of registration through 6 months after the final vaccine cycle; Note: adequate contraception methods include birth control pills, barrier device, intrauterine device
  • Willing to provide blood samples for correlative research purposes
  • Eastern Cooperative Oncology Group (ECOG) Performance Status (PS) 0 or 1
  • Willing to receive a tetanus vaccination if you have not had one within the past year

Exclusion Criteria:

  • Any prior lapatinib or pertuzumab treatment
  • Any of the following because this study involves an investigational agent whose genotoxic, mutagenic and teratogenic effects on the developing fetus and newborn are unknown:
    • Pregnant women
    • Nursing women unwilling to stop breast feeding
    • Men or women of child bearing potential who are unwilling to employ adequate contraception from the time of registration through 6 months after the final vaccine cycle
  • Co-morbid systemic illnesses or other severe concurrent disease which, in the judgment of the investigator, would make the patient inappropriate for entry into this study or interfere significantly with the proper assessment of safety and toxicity of the prescribed regimens
  • Immunocompromised patients including patients known to be human immunodeficiency virus (HIV) positive or those on chronic steroids
    • Note: must be off systemic steroids at least 90 days prior to registration
    • Topical steroids or steroid eye drops are permitted
  • Uncontrolled intercurrent illness including, but not limited to:
    • Ongoing or active infection
    • Symptomatic congestive heart failure
    • Unstable angina pectoris
    • Cardiac arrhythmia
    • Psychiatric illness/social situations that would limit compliance with study requirements
  • Uncontrolled acute or chronic medical conditions including, but not limited to the following:
    • Active infection requiring antibiotics
    • Congestive heart failure with New York Heart Association class III or IV
    • Moderate to severe objective evidence of cardiovascular disease
    • Myocardial infarction or stroke within previous 6 months
    • Receiving any other investigational agent
    • Other active malignancy at time of registration or within the last three years prior to registration. EXCEPTIONS:
      • Non-melanoma skin cancer 
      • Carcinoma-in-situ (eg of cervix, prostate)
      • NOTE: if there is a history of prior malignancy, they must not be receiving other specific treatment (cytotoxics, monoclonal antibodies, small molecule inhibitors) for their cancer
    • Known history of autoimmune disease, including Type I diabetes
    • Any prior hypersensitivity or adverse reaction to granulocyte-macrophage colony-stimulating factor (GM-CSF)
    • History of trastuzumab-related cardiac toxicity requiring interruption or discontinuation of therapy, even if left ventricular ejection fraction (LVEF) fully recovered Baseline LVEF with a value below 55%
    • Failure to fully recover from acute, reversible effects of prior chemotherapy regardless of interval since last treatment
    • History of myocardial infarction ≤ 168 days (6 months) prior to registration
    • Congestive heart failure requiring use of ongoing maintenance therapy for life-threatening ventricular arrhythmias
    • Bilateral invasive breast cancer, either synchronous or metachronous
      • Note: ductal carcinoma in situ in the contralateral breast is permissible

Participating Mayo Clinic locations

Study statuses change often. Please contact the study team for the most up-to-date information regarding possible participation.

Mayo Clinic Location Status Contact

Rochester, Minn.

Mayo Clinic principal investigator

Keith Knutson, Ph.D.

Closed for enrollment

Contact information:

Cancer Center Clinical Trials Referral Office

(855) 776-0015

More information


Publications are currently not available

Study Results Summary

Not yet available

Supplemental Study Information

Not yet available


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