Publications

  1. Chandra A, Lagnado AB, Farr JN, Monroe DG, Park S, Hachfeld C, Tchkonia T, Kirkland JL, Khosla S, Passos JF, Pignolo RJ. Targeted Reduction of Senescent Cell Burden Alleviates Focal Radiotherapy-Related Bone Loss. J Bone Miner Res. 2020 Jun; 35 (6):1119-1131 Epub 2020 Mar 05
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  2. Jayaraman S, Hou X, Kuffel MJ, Suman VJ, Hoskin TL, Reinicke KE, Monroe DG, Kalari KR, Tang X, Zeldenrust MA, Cheng J, Bruinsma ES, Buhrow SA, McGovern RM, Safgren SL, Walden CA, Carter JM, Reid JM, Ingle JN, Ames MM, Hawse JR, Goetz MP. Antitumor activity of Z-endoxifen in aromatase inhibitor-sensitive and aromatase inhibitor-resistant estrogen receptor-positive breast cancer. Breast Cancer Res. 2020 May 19; 22 (1):51
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  3. Eckhardt BA, Rowsey JL, Thicke BS, Fraser DG, O'Grady KL, Bondar OP, Hines JM, Singh RJ, Thoreson AR, Rakshit K, Lagnado AB, Passos JF, Vella A, Matveyenko AV, Khosla S, Monroe DG, Farr JN. Accelerated osteocyte senescence and skeletal fragility in mice with type 2 diabetes. JCI Insight. 2020 May 7; 5 (9) Epub 2020 May 07
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  4. Aquino-Martinez R, Rowsey JL, Fraser DG, Eckhardt BA, Khosla S, Farr JN, Monroe DG. LPS-induced premature osteocyte senescence: Implications in inflammatory alveolar bone loss and periodontal disease pathogenesis. Bone. 2020 Mar; 132:115220 Epub 2020 Jan 02
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  5. Weivoda MM, Chew CK, Monroe DG, Farr JN, Atkinson EJ, Geske JR, Eckhardt B, Thicke B, Ruan M, Tweed AJ, McCready LK, Rizza RA, Matveyenko A, Kassem M, Andersen TL, Vella A, Drake MT, Clarke BL, Oursler MJ, Khosla S. Identification of osteoclast-osteoblast coupling factors in humans reveals links between bone and energy metabolism. Nat Commun. 2020 Jan 7; 11 (1):87 Epub 2020 Jan 07
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  6. Farr JN, Rowsey JL, Eckhardt BA, Thicke BS, Fraser DG, Tchkonia T, Kirkland JL, Monroe DG, Khosla S. Independent Roles of Estrogen Deficiency and Cellular Senescence in the Pathogenesis of Osteoporosis: Evidence in Young Adult Mice and Older Humans. J Bone Miner Res. 2019 Aug; 34 (8):1407-1418 Epub 2019 June 21
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  7. Aquino-Martinez R, Farr JN, Weivoda MM, Negley BA, Onken JL, Thicke BS, Fulcer MM, Fraser DG, van Wijnen AJ, Khosla S, Monroe DG. miR-219a-5p Regulates Rorbeta During Osteoblast Differentiation and in Age-related Bone Loss. J Bone Miner Res. 2019 Jan; 34 (1):135-144 Epub 2018 Oct 15
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  8. Aquino-Martinez R, Monroe DG, Ventura F. Calcium mimics the chemotactic effect of conditioned media and is an effective inducer of bone regeneration. PLoS One. 2019; 14 (1):e0210301 Epub 2019 Jan 04
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  9. Khosla S, Drake MT, Volkman TL, Thicke BS, Achenbach SJ, Atkinson EJ, Joyner MJ, Rosen CJ, Monroe DG, Farr JN. Sympathetic beta1-adrenergic signaling contributes to regulation of human bone metabolism. J Clin Invest. 2018 Nov 1; 128 (11):4832-4842 Epub 2018 Oct 02
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  10. Reese JM, Bruinsma ES, Nelson AW, Chernukhin I, Carroll JS, Li Y, Subramaniam M, Suman VJ, Negron V, Monroe DG, Ingle JN, Goetz MP, Hawse JR. ERbeta-mediated induction of cystatins results in suppression of TGFbeta signaling and inhibition of triple-negative breast cancer metastasis. Proc Natl Acad Sci U S A. 2018 Oct 9; 115 (41):E9580-E9589 Epub 2018 Sept 26
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  11. Farr JN, Weivoda MM, Nicks KM, Fraser DG, Negley BA, Onken JL, Thicke BS, Ruan M, Liu H, Forrest D, Hawse JR, Khosla S, Monroe DG. Osteoprotection Through the Deletion of the Transcription Factor Rorbeta in Mice. J Bone Miner Res. 2018 Apr; 33 (4):720-731 Epub 2017 Dec 27
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  12. Subramaniam M, Pitel KS, Bruinsma ES, Monroe DG, Hawse JR. TIEG and estrogen modulate SOST expression in the murine skeleton. J Cell Physiol. 2018 Apr; 233 (4):3540-3551 Epub 2017 Nov 24
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  13. Khosla S, Monroe DG. Regulation of Bone Metabolism by Sex Steroids. Cold Spring Harb Perspect Med. 2018 Jan 2; 8 (1) Epub 2018 Jan 02
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  14. Reese JM, Bruinsma ES, Monroe DG, Negron V, Suman VJ, Ingle JN, Goetz MP, Hawse JR. ERbeta inhibits cyclin dependent kinases 1 and 7 in triple negative breast cancer. Oncotarget. 2017 Nov 14; 8 (57):96506-96521 Epub 2017 Oct 11
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  15. Farr JN, Xu M, Weivoda MM, Monroe DG, Fraser DG, Onken JL, Negley BA, Sfeir JG, Ogrodnik MB, Hachfeld CM, LeBrasseur NK, Drake MT, Pignolo RJ, Pirtskhalava T, Tchkonia T, Oursler MJ, Kirkland JL, Khosla S. Targeting cellular senescence prevents age-related bone loss in mice. Nat Med. 2017 Sep; 23 (9):1072-1079 Epub 2017 Aug 21
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  16. Stout MB, Steyn FJ, Jurczak MJ, Camporez JG, Zhu Y, Hawse JR, Jurk D, Palmer AK, Xu M, Pirtskhalava T, Evans GL, de Souza Santos R, Frank AP, White TA, Monroe DG, Singh RJ, Casaclang-Verzosa G, Miller JD, Clegg DJ, LeBrasseur NK, von Zglinicki T, Shulman GI, Tchkonia T, Kirkland JL. 17alpha-Estradiol Alleviates Age-related Metabolic and Inflammatory Dysfunction in Male Mice Without Inducing Feminization. J Gerontol A Biol Sci Med Sci. 2017 Jan; 72 (1):3-15 Epub 2016 Jan 24
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  17. Farr JN, Fraser DG, Wang H, Jaehn K, Ogrodnik MB, Weivoda MM, Drake MT, Tchkonia T, LeBrasseur NK, Kirkland JL, Bonewald LF, Pignolo RJ, Monroe DG, Khosla S. Identification of Senescent Cells in the Bone Microenvironment. J Bone Miner Res. 2016 Nov; 31 (11):1920-1929 Epub 2016 Oct 24
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  18. Nicks KM, Fujita K, Fraser D, McGregor U, Drake MT, McGee-Lawrence ME, Westendorf JJ, Monroe DG, Khosla S. Deletion of Estrogen Receptor Beta in Osteoprogenitor Cells Increases Trabecular but Not Cortical Bone Mass in Female Mice. J Bone Miner Res. 2016 Mar; 31 (3):606-14 Epub 2015 Oct 13
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  19. Roforth MM, Farr JN, Fujita K, McCready LK, Atkinson EJ, Therneau TM, Cunningham JM, Drake MT, Monroe DG, Khosla S. Global transcriptional profiling using RNA sequencing and DNA methylation patterns in highly enriched mesenchymal cells from young versus elderly women. Bone. 2015 Jul; 76:49-57 Epub 2015 Mar 27
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  20. Farr JN, Roforth MM, Fujita K, Nicks KM, Cunningham JM, Atkinson EJ, Therneau TM, McCready LK, Peterson JM, Drake MT, Monroe DG, Khosla S. Effects of Age and Estrogen on Skeletal Gene Expression in Humans as Assessed by RNA Sequencing. PLoS One. 2015; 10 (9):e0138347 Epub 2015 Sept 24
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  21. Fujita K, Xing Q, Khosla S, Monroe DG. Mutual enhancement of differentiation of osteoblasts and osteocytes occurs through direct cell-cell contact. J Cell Biochem. 2014 Nov; 115 (11):2039-44
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  22. Fujita K, Roforth MM, Atkinson EJ, Peterson JM, Drake MT, McCready LK, Farr JN, Monroe DG, Khosla S. Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture. Osteoporos Int. 2014 Mar; 25 (3):887-95 Epub 2013 Oct 11
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  23. Roforth MM, Fujita K, McGregor UI, Kirmani S, McCready LK, Peterson JM, Drake MT, Monroe DG, Khosla S. Effects of age on bone mRNA levels of sclerostin and other genes relevant to bone metabolism in humans. Bone. 2014 Feb; 59:1-6 Epub 2013 Oct 29
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  24. Roforth MM, Atkinson EJ, Levin ER, Khosla S, Monroe DG. Dissection of estrogen receptor alpha signaling pathways in osteoblasts using RNA-sequencing. PLoS One. 2014; 9 (4):e95987 Epub 2014 Apr 28
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  25. Fujita K, Roforth MM, Demaray S, McGregor U, Kirmani S, McCready LK, Peterson JM, Drake MT, Monroe DG, Khosla S. Effects of estrogen on bone mRNA levels of sclerostin and other genes relevant to bone metabolism in postmenopausal women. J Clin Endocrinol Metab. 2014 Jan; 99 (1):E81-8 Epub 2013 Dec 20
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  26. Roforth MM, Khosla S, Monroe DG. Identification of Rorbeta targets in cultured osteoblasts and in human bone. Biochem Biophys Res Commun. 2013 Nov 1; 440 (4):768-73 Epub 2013 Oct 11
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  27. Khosla S, Oursler MJ, Monroe DG. Estrogen and the skeleton. Trends Endocrinol Metab. 2012 Nov; 23 (11):576-81 Epub 2012 May 16
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  28. Chokalingam K, Roforth MM, Nicks KM, McGregor U, Fraser D, Khosla S, Monroe DG. Examination of ERalpha signaling pathways in bone of mutant mouse models reveals the importance of ERE-dependent signaling. Endocrinology. 2012 Nov; 153 (11):5325-33 Epub 2012 Sept 26
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  29. Farr JN, Charkoudian N, Barnes JN, Monroe DG, McCready LK, Atkinson EJ, Amin S, Melton LJ 3rd, Joyner MJ, Khosla S. Relationship of sympathetic activity to bone microstructure, turnover, and plasma osteopontin levels in women. J Clin Endocrinol Metab. 2012 Nov; 97 (11):4219-27 Epub 2012 Sept 04
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  30. Modder UI, Rudnik V, Liu G, Khosla S, Monroe DG. A DNA binding mutation in estrogen receptor-alpha leads to suppression of Wnt signaling via beta-catenin destabilization in osteoblasts. J Cell Biochem. 2012 Jul; 113 (7):2248-55
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  31. Roforth MM, Liu G, Khosla S, Monroe DG. Examination of nuclear receptor expression in osteoblasts reveals Rorbeta as an important regulator of osteogenesis. J Bone Miner Res. 2012 Apr; 27 (4):891-901
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  32. Modder UI, Roforth MM, Nicks KM, Peterson JM, McCready LK, Monroe DG, Khosla S. Characterization of mesenchymal progenitor cells isolated from human bone marrow by negative selection. Bone. 2012 Mar; 50 (3):804-10 Epub 2011 Dec 28
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  33. Monroe DG, McGee-Lawrence ME, Oursler MJ, Westendorf JJ. Update on Wnt signaling in bone cell biology and bone disease. Gene. 2012 Jan 15; 492 (1):1-18 Epub 2011 Nov 03
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  34. Modder UI, Roforth MM, Hoey K, McCready LK, Peterson JM, Monroe DG, Oursler MJ, Khosla S. Effects of estrogen on osteoprogenitor cells and cytokines/bone-regulatory factors in postmenopausal women. Bone. 2011 Aug; 49 (2):202-7 Epub 2011 Apr 29
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  35. Syed FA, Fraser DG, Monroe DG, Khosla S. Distinct effects of loss of classical estrogen receptor signaling versus complete deletion of estrogen receptor alpha on bone. Bone. 2011 Aug; 49 (2):208-16 Epub 2011 Mar 31
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  36. Modder UI, Oursler MJ, Khosla S, Monroe DG. Wnt10b activates the Wnt, notch, and NFkappaB pathways in U2OS osteosarcoma cells. J Cell Biochem. 2011 May; 112 (5):1392-402
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  37. Monroe DG, Hawse JR, Subramaniam M, Spelsberg TC. Retinoblastoma binding protein-1 (RBP1) is a Runx2 coactivator and promotes osteoblastic differentiation. BMC Musculoskelet Disord. 2010 May 28; 11:104
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  38. Modder UI, Monroe DG, Fraser DG, Spelsberg TC, Rosen CJ, Gehin M, Chambon P, O'Malley BW, Khosla S. Skeletal consequences of deletion of steroid receptor coactivator-2/transcription intermediary factor-2. J Biol Chem. 2009 Jul 10; 284 (28):18767-77 Epub 2009 May 07
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  39. Hawse JR, Subramaniam M, Monroe DG, Hemmingsen AH, Ingle JN, Khosla S, Oursler MJ, Spelsberg TC. Estrogen receptor beta isoform-specific induction of transforming growth factor beta-inducible early gene-1 in human osteoblast cells: an essential role for the activation function 1 domain. Mol Endocrinol. 2008 Jul; 22 (7):1579-95 Epub 2008 May 15
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  40. Hawse JR, Iwaniec UT, Bensamoun SF, Monroe DG, Peters KD, Ilharreborde B, Rajamannan NM, Oursler MJ, Turner RT, Spelsberg TC, Subramaniam M. TIEG-null mice display an osteopenic gender-specific phenotype. Bone. 2008 Jun; 42 (6):1025-31 Epub 2008 Mar 04
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  41. Rudnik V, Sanyal A, Syed FA, Monroe DG, Spelsberg TC, Oursler MJ, Khosla S. Loss of ERE binding activity by estrogen receptor-alpha alters basal and estrogen-stimulated bone-related gene expression by osteoblastic cells. J Cell Biochem. 2008 Feb 15; 103(3):896-907.
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  42. Secreto FJ, Monroe DG, Dutta S, Ingle JN, Spelsberg TC. Estrogen receptor alpha/beta isoforms, but not betacx, modulate unique patterns of gene expression and cell proliferation in Hs578T cells. J Cell Biochem. 2007 Aug 1; 101(5):1125-47.
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  43. Rajamannan NM, Subramaniam M, Abraham TP, Vasile VC, Ackerman MJ, Monroe DG, Chew TL, Spelsberg TC. TGFbeta inducible early gene-1 (TIEG1) and cardiac hypertrophy: Discovery and characterization of a novel signaling pathway. J Cell Biochem. 2007 Feb 1; 100(2):315-25.
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  44. Monroe DG, Secreto FJ, Hawse JR, Subramaniam M, Khosla S, Spelsberg TC. Estrogen receptor isoform-specific regulation of the retinoblastoma-binding protein 1 (RBBP1) gene: roles of AF1 and enhancer elements. J Biol Chem. 2006 Sep 29; 281(39):28596-604. Epub 2006 Jul 26.
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  45. Monroe DG, Secreto FJ, Khosla S, Spelsberg TC. The classical estrogen receptor transcriptional pathway: Implications in human osteoblasts. Clinical Reviews in Bone & Mineral Metabolism. 2006; 4(2):129-40.
  46. Effenberger KE, Johnsen SA, Monroe DG, Spelsberg TC, Westendorf JJ. Regulation of osteoblastic phenotype and gene expression by hop-derived phytoestrogens. J Steroid Biochem Mol Biol. 2005 Sep; 96(5):387-99. Epub 2005 Jul 12.
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  47. Monroe DG, Secreto FJ, Subramaniam M, Getz BJ, Khosla S, Spelsberg TC. Estrogen receptor alpha and beta heterodimers exert unique effects on estrogen- and tamoxifen-dependent gene expression in human U2OS osteosarcoma cells. Mol Endocrinol. 2005 Jun; 19(6):1555-68. Epub 2005 Mar 31.
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  48. Subramaniam M, Gorny G, Johnsen SA, Monroe DG, Evans GL, Fraser DG, Rickard DJ, Rasmussen K, van Deursen JM, Turner RT, Oursler MJ, Spelsberg TC. TIEG1 null mouse-derived osteoblasts are defective in mineralization and in support of osteoclast differentiation in vitro. Mol Cell Biol. 2005 Feb; 25 (3):1191-9
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  49. Monroe DG, Secreto FJ, Spelsberg TC. Overview of estrogen action in osteoblasts: role of the ligand, the receptor, and the co-regulators. J Musculoskelet Neuronal Interact. 2003 Dec; 3(4):357-62; discussion 381.
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  50. Monroe DG, Getz BJ, Johnsen SA, Riggs BL, Khosla S, Spelsberg TC. Estrogen receptor isoform-specific regulation of endogenous gene expression in human osteoblastic cell lines expressing either ERalpha or ERbeta. J Cell Biochem. 2003 Oct 1; 90(2):315-26.
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  51. Rickard DJ, Monroe DG, Ruesink TJ, Khosla S, Riggs BL, Spelsberg TC. Phytoestrogen genistein acts as an estrogen agonist on human osteoblastic cells through estrogen receptors alpha and beta. J Cell Biochem. 2003 Jun 1; 89(3):633-46.
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  52. Monroe DG, Johnsen SA, Subramaniam M, Getz BJ, Khosla S, Riggs BL, Riggs BL, Spelsberg TC. Mutual antagonism of estrogen receptors alpha and beta and their preferred interactions with steroid receptor coactivators in human osteoblastic cell lines. J Endocrinol. 2003 Mar; 176 (3):349-57
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  53. Monroe DG, Spelsberg TC. A case for estrogen receptors on cell membranes and nongenomic actions of estrogen. Calcif Tissue Int. 2003 Mar; 72(3):183-4. Epub 2003 Jan 15.
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  54. Johnsen SA, Subramaniam M, Monroe DG, Janknecht R, Spelsberg TC. Modulation of transforming growth factor beta (TGFbeta)/Smad transcriptional responses through targeted degradation of TGFbeta-inducible early gene-1 by human seven in absentia homologue. J Biol Chem. 2002 Aug 23; 277: (34)30754-9.
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  55. Monroe DG, Berger RR, Sanders MM. Tissue-protective effects of estrogen involve regulation of caspase gene expression. Mol Endocrinol. 2002 Jun; 16 (6):1322-31
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  56. Monroe DG, Sanders MM. The COUP-adjacent repressor (CAR) element participates in the tissue-specific expression of the ovalbumin gene. Biochim Biophys Acta. 2000 Dec 15; 1517(1):27-32.
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  57. Hagen SG, Monroe DG, Dean DM, Sanders MM. Repression of chick multidrug resistance-associated protein 1 (chMRP1) gene expression by estrogen. Gene. 2000 Oct 31; 257(2):243-9.
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  58. Monroe DG, Jin DF, Sanders MM. Estrogen opposes the apoptotic effects of bone morphogenetic protein 7 on tissue remodeling. Mol Cell Biol. 2000 Jul; 20(13):4626-34.
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  59. Monroe DG, Wipf LP, Diggins MR, Matthees DP, Granholm NH. Agouti-related maturation and tissue distribution of alpha-Melanocyte Stimulating Hormone in wild-type (AwJ/AwJ) and mutant (Ay/a,a/a) mice. Pigment Cell Res. 1998 Oct; 11(5):310-3.
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