Narrator: Gene expression analysis is a powerful tool for measuring biological processes that underlie disease progression and therapeutic effectiveness.
However, analyzing RNA using traditional technologies requires complex steps to convert RNA to cDNA which is then amplified and quantified as a proxy for the original RNA.
This process introduces variability which may lead to biased data and an inability to replicate results.
Why introduce and count cDNA errors when you can simply count the native RNA?
Direct detection using nCounter technology provides highly robust data across clinically relevant samples while reducing hands-on time and simplifying analysis.
nCounter technology gets you to reproducible results faster.
Using molecular barcode technology RNA is directly tagged with a capture probe and a reporter probe that are specific to the target of interest creating a unique target-probe complex.
After hybridization, excess probes are removed leaving only purified target-probe complexes.
These complexes are immobilized and aligned on the imaging surface.
The sample is then scanned by an automated fluorescence microscope where labeled barcodes are directly counted, and the data analyzed through an intuitive analysis software.
Molecular barcoding technology can be used with RNA, miRNA, protein, and DNA analytes.
Direct detection with nCounter technology provides several advantages:
- Highly Reproducible Data - Across multiple users and sites.
- Robust Performance - On FFPE samples.
- Single Tube Multiplexing - Up to 800 RNA targets in a single tube.
- Simple Protocol - Less than 15 minutes of hands-on time.
- Flexible Sample Input.
- Simple Data Analysis - Publication ready figures in hours.
Faster than NGS. Simpler than qPCR.
Direct detection with nCounter technology by NanoString.