Identifying protein interactions in intracellular signaling
Dr. Sieck and his colleagues use confocal microscopy to image and characterize protein-protein interactions involved in intracellular signaling. In this example, the real-time interaction of mitochondria (labeled with MitoTracker Red) and F-actin (labeled with LifeAct — green) is shown in a human airway smooth muscle cell.
The Mayo Clinic Cell and Regenerative Physiology Laboratory, led by Gary C. Sieck, Ph.D., studies the physiology of muscles, specifically exploring neural control of contraction and cell signaling mechanisms involved in excitation-contraction coupling and mitochondrial energetic support.
Dr. Sieck's Cell and Regenerative Physiology Laboratory has developed an extensive array of state-of-the-art physiological, biomedical engineering and imaging techniques to explore physiological mechanisms. These techniques include:
- Confocal imaging
- Laser-capture microdissection and in situ hybridization for analysis of mRNA expression in single identified cells
- Biomechanical measurements of muscle performance
- Measurement and manipulation of protein expression
- Viral-mediated gene transfer in targeted cells
- Real-time functional imaging
- Exploration of cell signaling pathways
About Dr. Sieck
Dr. Sieck is a professor of physiology at Mayo Clinic School of Medicine and Science and a consultant in the Department of Physiology and Biomedical Engineering with joint appointments in the Department of Anesthesiology and Perioperative Medicine and the Department of Physical Medicine and Rehabilitation Medicine. Dr. Sieck's research focuses on muscle physiology and cell signaling. He has published more than 380 peer-reviewed papers, and his research has been continuously funded by multiple grants from the NIH for more than 37 years.