Equipment and capabilities
Mayo Clinic investigators have access to this equipment and these technologies in the RNA Therapeutics Discovery and Translation recharge facility:
Oligonucleotide Synthesis
| Equipment |
Manufacturer |
Primary Function |
| Dr. Oligo 192XLc+ |
Biolytic Lab Performance, Inc. |
DNA/RNA oligonucleotide synthesis system |
| ÄKTA avant 25 |
Cytiva |
High-performance liquid chromatography for oligonucleotide purification |
| 1290 Infinity II |
Agilent Technologies Inc. |
Liquid chromatography-mass spectrometry for oligonucleotide characterization |
| timsTOF Pro 2 |
Bruker Corp. |
Liquid chromatography-mass spectrometry for oligonucleotide characterization |
Messenger RNA (mRNA) Synthesis
| Equipment |
Manufacturer |
Primary Function |
| VeritiPro Thermal Cycler (96 well) |
Applied Biosystems |
Thermal cycling for enzymatic reactions |
| QuantStudio 5 |
Thermo Fisher Scientific Inc. |
Real-time polymerase chain reaction for nucleic acid analysis |
| 4150 TapeStation |
Agilent Technologies Inc. |
DNA-RNA quality control |
| 5200 Fragment Analyzer |
Agilent Technologies Inc. |
DNA-RNA quality control |
| Qubit™ 4 Fluorometer |
Thermo Fisher Scientific Inc. |
Nucleic acid quantification |
| BioXP 3250 |
Telesis Bio Inc. |
Automated mRNA synthesis |
| NanoDrop One |
Thermo Fisher Scientific Inc. |
Nucleic acid spectrophotometry |
Lipid Nanoparticle Synthesis
| Equipment |
Manufacturer |
Primary Function |
| NanoGenerator Flex S |
Precigenome LLC |
Small-scale nanoparticle synthesis |
| Sunshine |
Unchained Labs |
Large-scale nanoparticle synthesis |
| ZetaSizer Pro |
Malvern Panalytical Ltd. |
Nanoparticle size, polydispersity index, zeta-potential |
| ZetaView Quatt |
Particle Metrix GmbH |
Nanoparticle tracking analysis; nanoparticle size and concentration |
| ARC |
Spectradyne Particle Analysis |
Nanoparticle size and concentration / microfluidic resistive pulse sensing |
Shared/Cross-Cutting Infrastructure
| Equipment |
Manufacturer |
Primary Function |
| OPTIMA AUC |
Beckman Coulter Inc. |
Analytical ultracentrifuge |
| CS150FNX |
Eppendorf SE |
Small-volume ultracentrifuge |
| Micro 21R |
Thermo Fisher Scientific Inc. |
Refrigerated benchtop centrifuge |
| MySPIN 6 |
Heathrow Scientific |
Microcentrifuge |
| FLUOstar Omega |
BMG Labtech |
Microplate reader (absorbance, luminescence, fluorescence) |
| Thermal Mixer |
Thermo Fisher Scientific Inc. |
Sample incubation and mixing |
| ExPERT GTx |
MaxCyte |
Electroporation system |
| XPR105 |
Mettler Toledo |
Analytical scale |
| Scout |
Ohaus Corp. |
Benchtop scale |
| Lab Star PH111 |
Orion a |
pH meter |
| MDF DU302VX PA (−80 °C) |
PHCbi |
Ultra-low-temperature freezer |
| MPR N250FSH PA (4 °C / −20 °C) |
PHCbi |
Laboratory refrigerators and freezers |
Scheduling
Some equipment is self-serve and can be reserved. Equipment can be reserved using the Research Device Scheduling System and selecting "JaxRNA" in the Area of Interest drop-down menu.
You need to be logged in to the Mayo network to access the scheduler. You need to have preauthorization to use the scheduling system and be properly trained on the equipment to reserve it.