C. Elegans PKD-Targeted Services
The Model Systems Core provides services using C. elegans to PKD researchers both at Mayo Clinic and at other institutions.
Because it's difficult to study cilia biology and the connections among cilia formation, sensory function and disease in mammalian model organisms, alternative experimental systems such as C. elegans are necessary.
With its evolutionarily conserved PKD genes, polycystin ciliary localization and polycystin sensory function, C. elegans is a powerful model for characterizing the physiological roles of PKD genes in their native cellular environment.
The Model Systems Core offers five C. elegans PKD-targeted services:
- Small-molecular screening for cystic phenotype effects, such as mating behavior and dye filling
- Access to worm mutants involved in the polycystin signaling pathway
- Analysis of genetic, molecular and cellular interactions of evolutionarily conserved candidate genes with polycystins and other components involved in polycystin signaling
- Screens for deletion mutants of worm homologues of interesting mammalian genes
- Training on C. elegans-related technologies, including cilia structure and function, worm genetics, and whole-genome mutagenesis and high-throughput RNAi screenings
The Model Systems Core offers dozens of PKD-related mutants and GFP-tagged transgenic animals expressing the polycystins and other ciliary markers frozen in the core.
Email us for more information about C. elegans PKD services and available lines.