Services and Assays

The Metabolomics Core:

Develops stable isotope methodologies and makes them available to clinical investigators across various disciplines
Provides advice and expertise in the application of stable isotope-labeled tracer techniques
Offers measurement of metabolic profiles, both in terms of metabolite identification and quantification

Assays available in the core and those under development include:

Assay	Medium
Mass spectrometry (gases)
CO₂	Expired air
Doubly labeled water	Urine
Deuterium	Urine
Deuterium	Plasma
Gas chromatography mass spectrometry (GC/MS)
[6,6-d₂]glucose	Plasma
[1-¹³C]glucose	Plasma
[6,6-d₂]glucose plus [1-¹³C]glucose panel	Plasma
[¹³C₂]oxalate	Urine
[d₂]d-ribose	Cells
[d₅]glycerol	Plasma
[¹³C₃]glycerol	Plasma
Liquid chromatography with tandem mass spectrometry (LC-MS/MS)
[ring-¹³C₆]phenylalanine	Plasma
[ring-¹³C₆]tyrosine	Plasma
[¹⁵N]tyrosine	Plasma
[d₅]phenylalanine	Plasma
[ring-¹³C₆]phenylalanine	Tissue fluid
[d₅]phenylalanine	Tissue fluid
[d₄]cortisol plus cortisone panel	Plasma
[4-¹³C]cortisol plus [d₇]cortisone panel	Plasma
[ring-¹³C₆]phenylalanine	Gel spots
[d₅]phenylalanine	Gel spots
[U-¹³C]palmitate	Plasma
[U-¹³C]oleate	Plasma
Ceramide plus metabolites	Plasma or tissue
Free fatty acid (FFA) panel	Plasma
[U-¹³C]palmitoyl carnitine	Muscle
[¹³C₄]palmitoyl carnitine	Muscle
[U-¹³C]oleoyl carnitine	Muscle
8-oxo-dG	DNA
Large-scale metabolites profiling	Plasma or tissue
Diazepam plus metabolites panel	Media
Gas chromatography combustion isotope ratio mass spectrometry (GC-C-IR/MS)
[U-¹³C]palmitate	Plasma
[U-¹³C]oleate	Plasma
Under development
Metabolites identification	Plasma or tissue
Isoprostanes	Plasma
Citric acid flux	Tissue
Diacylglycerol (DAG)	Plasma or tissue
Glutamic acid flux	Plasma
Arachidonic acid plus metabolites	Plasma or tissue