The Metabolomics Nuclear Magnetic Resonance Spectroscopy (NMRS) Core provides an NMRS-based dynamic metabolomic platform for untargeted and targeted quantification of metabolite levels and measurement of fluxes within metabolic networks in human and transgenic animal biofluids. Metabolomic profiling is performed using:
- Whole blood, plasma, cerebrospinal fluid and urine
- Tissue samples such as heart, brain and atrial appendage
- Cell cultures in vitro and in vivo
The Metabolomics NMRS Core offers expertise in standard high-throughput 1H and 31P NMR quantitative metabolomics measurements. It also provides advanced metabolomics services using novel 18O-assisted 31P NMRS- and high-performance liquid chromatography/gas chromatography/mass spectrometry (HPLC/GC/MS)-based methodology for simultaneously measuring phosphometabolite levels, turnover rates, metabolic fluxes, and 13C NMRS-based isotopomer tracer analysis of carbon flux in metabolic networks and flux analysis through different metabolic pathways.
The Metabolomics NMRS Core and Metabolomics Mass Spectrometry Core are integral parts of the Mayo Clinic Metabolomics Resource Core (MCCRC). They work together to implement orthogonal metabolite identification, metabolic flux (18O and 13C labeling) analysis and metabolite structure characterization services. The cores' robust in vivo spectroscopy methods include 1H, 31P volume-selective NMRS and NMRS imaging for analyzing metabolite levels in intact organs such as brain, heart, kidney and muscle.
The Metabolomics NMRS Core also works with the Bioinformatics Core, Proteomics Core, Molecular Biology Core and genomics cores to provide an integrated approach to omics-related research for investigators inside and outside Mayo Clinic.
Mayo Clinic receives $8.8 million federal grant for Metabolomics Center
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This publication was made possible by Mayo Clinic Metabolomics Resource Core through grant number U24DK100469 from the National Institute of Diabetes and Digestive and Kidney Diseases and originates from the National Institutes of Health Director's Common Fund.