Authentication of Resources

The Mayo Clinic Brain Tumor Patient-Derived Xenograft National Resource can provide a sample paragraph that can be used as part of a National Institutes of Health (NIH) application section relating to authentication of resources, which might be required as part of an NIH grant application.

Here's a sample paragraph:

Patient-derived xenograft models were developed by Dr. Sarkaria's Translational Neuro-Oncology Laboratory at Mayo Clinic. Short-tandem repeat (STR) was used to compare the original patient tumor sample, when available, with the derivative PDX model to ensure the provenance of the PDX line. STR analysis from early passage PDX tissue was used to establish a gold standard identity for each PDX line, and these data are publicly available. To ensure provenance of the associated molecular analyses performed by Dr. Sarkaria's laboratory, DNA submitted for whole-exome sequencing (WES) was analyzed by STR to ensure that the DNA submitted was for the correct PDX line. A sample ID validation panel was performed on the DNA submitted for sequencing and compared with the WES data to prevent delivery of misidentified sequencing data. In support of the proposed work, STR analysis will be performed on a semiannual basis to confirm the identity of each PDX line that is used. Moreover, an aliquot of tumor cells from each animal experiment will be frozen and archived to facilitate STR confirmation of tumor identity for all animal studies.