Images

  • Microscopy of hepatocyte spheroids demonstrating configuration of cells in 3-D aggregates
    Microscopy of hepatocyte spheroids demonstrating configuration of cells in 3-D aggregates
  • Spheroid Reservoir Bioartificial Liver circuit.
    Spheroid Reservoir Bioartificial Liver circuit
  • Overview of the approach for production of human hepatocytes in FAH-deficient pigs.
    Overview of the approach for production of human hepatocytes in FAH-deficient pigs

    Overview of the approach for production of human hepatocytes in FAH-deficient pigs. Step one involves in utero cell transplantation of donor cells into fetal FAH-deficient pigs. Step two involves tapering off the protective nitisinone drug with expansion of donor cells. Step three involves digestion of the humanized liver and enrichment of expanded human cells. The potential clinical applications of human hepatocytes include a humanized bioartificial liver, an issue engineered human liver and human hepatocyte transplantation.

  • MR Elastography demonstrating increased liver stiffness of FAH-deficient pigs.
    MR elastography demonstrating increased liver stiffness of FAH-deficient pigs

    MR elastography demonstrating increased liver stiffness of homozygote FAH-deficient pigs maintained on low dose of nitisinone. These images suggest that homozygote FAH-deficient pigs may also serve as a spontaneous model of portal hypertension and liver cirrhosis.

  • Hepatocyte spheroids maintain mature phenotype for at least 28 days in culture.
    Hepatocyte spheroids maintain mature phenotype for at least 28 days in culture