Protein Lysates

For a subset of commonly used patient-derived xenograft lines in the Mayo Clinic Brain Tumor Patient-Derived Xenograft National Resource, three biological replicate flank tumor samples for each PDX line were lysed in an isotonic NP40-based lysis buffer, and equal amounts of protein were pooled from each tumor.

The pooled lysate was combined with 4X SDS sample buffer and stored at minus 80 degrees Celsius.

Files available:

Aliquots suitable for Western blotting also are available. To request aliquots, contact the PDX National Resource.