Hai-Long Wang

Email: wang.hai-long@mayo.edu

Education

  • FuDan University, Shanghai, China
    B.S. - Biophysics, 1987
  • Shanghai Institute of Physiology, Chinese Academia of Science
    Ph.D. - Neurobiology, 1994
  • Professional experience

  • Practice Researcher, Shanghai Institute of Cell Biology, Chinese Academia of Science 7/87 - 7/89
  • Postdoctoral Research Fellow, Department of Physiology and Biophysics, Mayo Foundation, Rochester MN 1/95 - 1/98
  • Senior Research Fellow, Department of Physiology and Biophysics,
  • Mayo Foundation, Rochester MN 1/98 - 1/2001
  • Research Associate, Department of Physiology and Biophysics,
  • Mayo Foundation, Rochester MN 1/01-1/2002
  • Senior Research Associate, Department of Physiology and Biophysics,
  • Mayo Foundation, Rochester MN 1/02-present

Publications

  • Wang H-L., Wei J-Y and Yang X-L. Suppression of Carp retinal bipolar cells in darkness. Chinese Science Bulletin 39 (2): 158-161, 1994
  • Wang H-L., Wei J-Y., and Yang X-L. Centrifugal fibers to the retina. Sheng Li Ko Hsueh Chin Chan 25, 22-26. 1994. (review)
  • Wei J-Y., Wang H-L., Yang X-L. Effects of acute hypoxia on response of horizontal cells and electroretinograms in carp retina in vivo. Chinese Science Bulletin . 1995
  • Wang H-L., Wei J-Y and Yang X-L. Effect of optic nerve stimulation on bipolar cell in the carp retina. Chinese Science Bulletin 40(5): 422-426, 1995
  • Wei J-Y., Wang H-L., and Yang X-L. Novel GABAc receptors. Sheng Li Ko Hsueh Chin Chan 26, 7-11. 1995. (review)
  • Wang H-L. and Yang X-L. Differential effects of low calcium on signal transmission from rods and cones to horizontal cells in carp retina. Sheng Li Hsueh Pao 48, 113-124. 1996.
  • Ohno, K., H. -L. Wang, M. Milone, N. Bren, J. M. Brengman, S. Nakano, P. Quiram, J.N. Pruitt, S. M. Sine and E. G. Engel. Congential myasthenic syndrome caused by decreased agonist binding affinity due to a mutation in the acetylcholine receptor e subunit. Neuron 17:157-170, 1996.
  • Engel, A. G., K. Ohno, M. Milone, H. -L. Wang, S. Nakano, C. Bouzat, J. N. Pruitt II, D. O. Hutchinson, J. M. Brengman, N. Bren, J. P. Sieb and S. M. Sine: New mutations in acetylcholine receptor subunit genes reveal heterogeneity in the slow-channel congenital myasthenic syndrome. Human Molecular Genetics 5: 1217-1227, 1996.
  • Ohno, K., Quiram, P., Milone, M., Wang, H-L., Harper, M., Pruitt, J., Brengman, J., Pao, L., Fischbeck, K., Crawford, T., Sine, S. and A. Engel. Congenital myasthenic syndromes due to heteroallelic nonsense/missense mutations in the acetylcholine receptor e subunit gene: identification and functional characterization of six new mutations. Human Molecular Genetics 6: 753-766, 1997.
  • Wang, H-L., Auerbach, A., Bren, N., Ohno, K., Engel, A., and S. Sine. Mutation in the M1 domain of the acetylcholine receptor a subunit decreases the rate of agonist dissociation. Journal of General Physiology 109: 757-766, 1997.
  • Milone, M., Wang, H-L., Ohno, K., Fukudome, T., Pruitt, N., Bren, N., Sine, S., and A. Engel. Slow channel myasthenic syndrome caused by enhanced activation, desensitization, and agonist binding affinity due to mutation in the M2 domain of the acetylcholine receptor a subunit. Journal of Neuroscience 17: 5651-5665, 1997.
  • Wang, H-L., Milone, M., Ohno, K., Prince, R., Fukudome, T., Shen, X-M., Brengman, J., Griggs, R., Sine, S. M., and A. Engel. Mode switching kinetics by a naturally occurring mutation in the cytoplasmic loop of the human acetylcholine receptor e subunit. Neuron 20: 575-588, 1998.
  • Engel, A., K. Ohno, H-L. Wang, M. Milone and S. M. Sine. Molecular basis of congenital myasthenic syndromes: mutations in the acetylcholine receptor. The Neuroscientist 4, 185-194, 1998
  • Wang, H.-L., Milone, M., Ohno, K., Shen, X.-M., Tsujuno, A., Paola, A., Tonali, P., Brengman, J., Engel, A. G. and Sine, S. M. Acetylcholine receptor M3 domain: stereochemical and volume contributions to channel gating. Nature Neuroscience 2, 226-233, 1999.
  • Wang, H.-L, Ohno, K., Milone, M., Brengman, J. Evoli, A., Batocchi, A. P., Middleton, L., Christodoulou, K., Engel, A. G. and Sine, S. M. Fundamental gating mechanism of nicotinic receptor channel gating revealed by mutation causing a congenital myasthenic syndrome. J. Gen. Physiol., 116, 449-460, 2000.
  • Iba¯ez-Tallon, I., Miwa, J., Wang, H.L., Adams, N., Crabtree, G., Sine, S. M. and Heintz, N. Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1. Neuron 33,893-903,2002.
  • Sine, S. M., Engel, A. G., Wang, H.-L., and Ohno, K. Molecular insights into acetylcholine structure and function revealed by mutations causing congenital myasthenic syndromes. In: R. Maue (Ed.) Molecular Insights into Ion Channel Biology in Health and Disease. Elsevier Science (In press).
  • Sine, S. M., Shen, X. M., Wang, H. L., Brengmann, J., Lee, W. Y. Bren, N. and Engel, A. G. State-specific contributions to agonist affinity revealed by naturally-occurring mutations in the human acetylcholine receptor epsilon subunit (In press JGP).
  • Sine. S. M., Wang, H.-L. and Bren, N. Lysine scanning mutagenesis delineates structure of nicotinic receptor ligand binding domain (In press JBC).

Abstracts

  • Ohno, K., Wang, H.-L., Milone, M., Bren, N., Brengman, J. M., Nakano, S., Quiram, P., Pruitt, J. N., Sine, S. M. and Engel, E. G. Congential myasthenic syndrome caused by decreased agonist-binding affinity due to a mutation in the acetylcholine receptor e subunit. Biophysical Journal, 1995, 70, p. A372.
  • Wang, H.-L., Bren, N., Auerbach, A., Engel, A. G.and Sine, S. M. Mutation in the M1 domain of the acetylcholine receptor a subunit decreases both the rate of agonist dissociation and rate constants for channel gating. Society for Neuroscience, 1996, 22, p. 501.5.
  • Ohno, K., Quiram, P., Milone, M., Wang, H-L., Harper, C. M., Pruitt, J., Brengman, J., Pao, L., Fishbeck, K., Crawford, T. O., Sine, S. M. and Engel, A. G. Congenital myasthenic syndromes due to heteroallelic nonsense/missense mutations in the acetylcholine receptor e subunit gene: Report of 5 new mutations. Neurology, 1997, 48, A73.
  • Ohno, K., Wang, H-L., Shen, X-M. Milone, M., Bernasconi, L., Sine, S. M. and Engel, A. G. Slow channel mutations in the center of the M1 transmembrane domain of the acetylcholine receptor a subunit. Neurology (Suppl 3), 1999, 54, A183.
  • Wang, H-L. and Sine, S. M. Hidden Markov modeling analysis reveals kinetic diversity of single acetylcholine receptor channel currents. Society for Neuroscience, 1999, 25, 1721.
  • Fan Gao, Hai-Long Wang, Steven M. Sine. Dynamic simulation and ligand docking study of Acetylcholine receptor ligand binding domain.

Professional societies

  • Society for Neuroscience

Grant awards

  • Fellowship from Myasthenia Gravis Foundation 1998.