DNA Flexibility in Cells
We are interested in understanding how the locally-stiff DNA double helix is managed within cells so that it can be folded, bent and looped during packaging and gene expression. In ongoing fundamental research projects, we are studying whether electrostatic (charge-charge) interactions are sufficient to explain how some proteins induce DNA to bend.
In particular, we are curious whether neutralization of negative charges along one face of DNA can cause the molecule to bend by collapse. In related studies, we are exploring the mechanisms of proteins that cause DNA to become more flexible. One focus is on the HMGB proteins that bind DNA without sequence preference. We wish to better understand the mechanism of these proteins and how they assist DNA looping in living cells.