Facilities

The Computer-Aided Molecular Design laboratory of Yuan-Ping Pang, Ph.D., is located on the 12th floor of the Stabile Building on the Mayo Clinic campus in Rochester, Minn. Laboratory areas include:

  • A computational chemistry unit (1,342 square feet)
  • A synthetic chemistry unit with 14 6-foot fume hoods (3,002 square feet)
  • A bioassay lab (88 square feet)

Equipment

Equipment for synthesis and characterization of compounds includes:

  • Varian 400 MHz nuclear magnetic resonance (NMR) system dedicated to Dr. Pang's lab, featuring a 1H/19F/X 5 mm autotune dual broadband probe, 1H-19F[15N-31P] 3 mm indirect broadband probe, 23 channel shims and shim supply, variable temperature module, and 100-sample autosampler
  • Thermo Nicolet Avatar 370 Fourier transform infrared spectrometer (FT-IR)
  • Jasco DIP-370 digital polarimeter
  • Molecular Devices SpectraMax Plus384 microplate reader
  • Three high-performance liquid chromatography (HPLC) systems (Beckman System Gold 125P solvent module and 166P UV detector)
  • Agilent HP 6890 gas chromatograph and Agilent HP5973 mass spectrometer
  • Liquid chromatography-mass spectrometry system (Waters 1525 binary HPLC pump, Waters 2487 dual lambda absorbance detector and Waters Micromass ZQ 2000)
  • Four Biotage SP1 flash purification systems
  • Isco CombiFlash Sg 100 gradient chromatography system
  • Isotemp cooling circulator (minus 20 degrees Celsius to 20 degrees Celsius)
  • Microcentrifuge, ovens, Kugelrohr distillation apparatus, melting point apparatus, hydrogenator, high-pressure reaction vessels, rotary evaporators, analytical balances, semi-micro balance, ozone generators, temperature controllers and freeze dryer

Equipment for solid-phase combinatorial chemistry using the radiofrequency-encoded split-and-pool technique includes:

  • AccuTag-100 system (AccuTag scanning station, Synthesis Manager software and MicroKan decapping tool)
  • Forty-eight-position optical sorting indicators
  • MultiTag-96 station drops radiofrequency tags into 8x12 arrays of MicroKans or MiniKans
  • MultiCap-96 station
  • Ninety-six-position dry resin filling station for MicroKans and MiniKans
  • Reuseable glass-coated radio frequency tags
  • MicroKans, MiniKans and MacroKans reactors
  • Genevac DD4 evaporation system

Equipment for parallel synthesis includes:

  • Argonaut Quest 210 ASW (equipped for solution- and solid-phase parallel synthesis) with automated solvent wash module and chiller interface kit
  • Five mL and 10 mL Teflon reaction vessels with 7 mm and 30 mm frits
  • JULABO FP88-MW compact ultra-low refrigerated circulator

Equipment for zebrafish experiments includes:

  • Leica M165 FC fluorescent stereo microscope with Leica IC80 HD camera, KL 1500 LCD and ErgoTube 10-50 binocular
  • Narishige three-axis course manipulator
  • Eppendorf Femtojet microinjector
  • Tecniplast ZEBTEC zebrafish housing system with 50 3.5-liter tanks, 15 1.1-liter tanks, a dedicated water treatment unit with automatic pH and conductivity dosing
  • Two 100-liter fish tanks (each equipped with one Eheim Classic 2217 external canister filter)
  • Noldus EthoVision XT video tracking system for tracking and analyzing the behavior, movement and activity of animals

Equipment for biochemistry and molecular biology includes:

  • Benchmark Scientific MyFuge 12 Mini Centrifuge
  • Labnet Prism R Refrigerated Microcentrifuge
  • Fisher Scientific Marathon Micro A Centrifuge
  • Fisher Scientific Centrific Model 228 Benchtop Centrifuge
  • Genevac DD-4 centrifugal evaporation system
  • Thermo Scientific Owl EasyCast B1 Mini Gel Electrophoresis System combs
  • Bio-Rad Mini-Protean Tetra Electrophoresis System
  • Bio-Rad MicroPulser electroporator
  • Fisher Scientific FB300 power supply
  • Bio-Rad PowerPac Basic power supply
  • Beckman Coulter ProteomeLab FC/1 module fraction collector
  • Fisher Scientific Isotemp Model 202S digital control water bath
  • DPC MicroMix Model 5 microplate and tube shaker
  • Invitrogen Sample Mixer
  • Bioer GenePro thermal cycler
  • Eppendorf Research pipettes (sets of 10, 20, 50, 100, 200 and 1000 microliters) and Biohit electronic eLine pipettes
  • Summit Commercial Products SCR600L Plus medical refrigerator with glass door
  • Molecular Devices SpectraMax Gemini XS microplate spectrofluorometer
  • Molecular Devices SpectraMax Plus384 microplate reader
  • Biacore 3000 Surface plasmon resonance biomolecular binding system

Dedicated computer hardware includes:

  • Kibbutz100 (1.1 teraflops, 470 Xeon processors, 2.2/2.4 GHz, 512 MB memory/node, 8.4 TB disk)
  • SilverGene (2.7 teraflops, 590 G5 processors, 2.0/2.3 GHz, 512 MB memory/node, 4 TB Xserve RAID)
  • PowerGene (IBM SMP workstation, four POWER5 processors, 1.95 GHz, 36 MB cache, 8 GB DDR2 memory)
  • LeopardGene (eight eight-core Mac Pros with Intel Woodcrest 3.0 GHz)
  • LionGene (30.0 teraflops, 100 12-core Mac Pros with Intel Westmere 2.40/2.93 GHz)