X-ray crystallography is the most powerful method for determining 3-D structures of biological macromolecules — such as proteins and nucleic acids — and their complexes with other macromolecules or ligands, substrates and inhibitors. It is the fastest, least expensive and most successful method in structural biology — no single experimental technique produces more-detailed data about a macromolecule.
The atomic structures resulting from X-ray crystallography are crucial for understanding the biochemical and biophysical roles of a macromolecule in cellular function and disease. This understanding comes through the study of physiochemical interactions between molecules, as well as the study of conformational changes and chemical catalysis.
X-ray crystallography has been utilized in major scientific advances during the past century — it's the means by which the molecular structures of DNA, hemoglobin, ion channels and RNA polymerase were determined. Additionally, nearly all recent Food and Drug Administration-approved small-molecule therapeutics depended upon crystallographic structures for decision making during drug discovery and development.
Crystallography at Mayo Clinic
Collaborations in the X-ray Crystallography Core at Mayo Clinic are using crystallography for studies related to rational therapeutic drug design, alterations in supramolecular structure, enzyme mechanisms, and protein and nucleic acid recognition.
Human health-related areas being investigated using crystallography at Mayo include:
- Renal Ca2+ reabsorption
- Membrane ion transport
- Lipoprotein assembly and secretion
- Cell-cycle regulation
- Light-chain amyloidosis
- Function of hemolytic toxins
- Chronic pain control
- Ca2+ regulation of blood clotting
- Neuromuscular degeneration
- DNA repair and cancer
- Bone growth regulation
- Obesity and diabetes