Services

A combination of orthogonal techniques is needed to obtain comprehensive information about the metabolome and enhance researchers' confidence in metabolite identification and detection of changes.

High-resolution metabolomics nuclear magnetic resonance spectroscopy (NMRS) is a stable, quantitative and reliable method that can detect proton-, phosphorus- and carbon-containing metabolites present in a sample.

Stable isotope-based metabolic flux analysis enables quantification of metabolite levels and turnover rates, which increases researchers' understanding of alterations in metabolic pathways and networks. It is not possible to quantify the contribution and dynamics of different pathways based only on total concentration of metabolites. NMRS is especially suitable for this analysis because it allows determination of positional isotopomer distribution derived from precursors enriched with stable isotopes. Isotope-edited 2-D NMRS experiments allow identification and quantification of more than 200 metabolites and isotopomers.

NMRS metabolic services

Metabolomic profiling and fingerprinting

  • Experimental design, sample preparation and choice of complementary metabolomic technologies
  • Data processing, metabolite identification and quantitation, and multivariate analysis and interpretation of metabolomic changes
  • NMR method: multinuclear (1H, 13C, 31P) 1-D and 2-D NMR
  • Specimen: biofluids, including plasma, blood, urine, CSF, and tissue and cell extracts

Targeted metabolomics of specific metabolite classes

  • Experimental design, sample preparation and choice of complementary metabolomic technology for specific metabolite class
  • Data processing, metabolite identification and quantitation, and multivariate analysis and interpretation of metabolomic changes
  • NMR method: multinuclear (1H, 2H, 13C, 15N, 31P) 1-D and 2-D NMR, and stable isotope-enhanced NMR
  • Specimen: biofluids such as tissue and cell extracts, in vivo, ex vivo and in vitro

Stable isotope tracer analysis, metabolite dynamics and flux (fluxomics)

  • Experimental design, stable isotope labeling, sample preparation and choice of complementary metabolomic technology for specific metabolic pathway
  • NMR in conjunction with high-performance liquid chromatography (HPLC) fractionation and mass spectrometry via the Metabolomics Mass Spectrometry Core
  • Data processing, metabolite identification and quantitation, calculation of labeling rates, multivariate and network analysis, and interpretation of metabolic flux changes
  • NMR method: 2H, 13C, 18O-assisted 31P 1-D and 2-D
  • Specimen: labeled tissue and cell extracts and biofluids, in vivo, ex vivo and in vitro

Structure elucidation of unknown metabolites

  • Experimental design, metabolite fractionation and concentration
  • Data analysis using metabolite structure elucidation and prediction software
  • NMR method: multinuclear, multidimensional NMR using 1.7 mm triple resonance NMR MicroCryoProbe
  • Specimen: isolated metabolite solution

In vivo NMRS and metabolite topological distribution in tissues and organs

  • Experimental design, animal handling for NMRS in vivo; and isolated organ and cell culture perfusion
  • Data processing, metabolite identification and quantitation, multivariate analysis and interpretation of metabolomic changes
  • NMR method: 1H and 31P localized 1-D and 2-D NMR spectroscopic imaging
  • Specimen: mouse, rat, human organs, cell cultures in vivo and ex vivo

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