Benchtop flow cytometry
The Flow Cytometry and Confocal Microscopy Core has three bench-top instruments: two Beckman Coulter Cyan ADP Analyzer flow cytometers and a BD Biosciences LSRFortessa cell analyzer. Typical applications include apoptosis, cell cycle, immunophenotyping and protein expression.
The core has a BD Biosciences FACS Aria III cell sorter. It is contained within a Baker biological safety cabinet to allow sterile sorting of both human and nonhuman cells. The cell sorter is equipped with an automatic cell deposition unit to enable sorting into various size plates, as well as glass slides and tubes. Four distinct populations can be sorted at one time.
A Zeiss LSM 510 confocal microscope is available for use by trained users. The setup includes a Zeiss Axiovert 200m inverted microscope contained within an incubation chamber supplied with heat and carbon dioxide. The core has six objectives with three dry (10x, 20x and 40x) and three oil (40x, 63x and 100x). The software used is the Zeiss LSM 510 Image Browser, which is able to distinguish between three different fluorescent dyes (three channels) and overlay them.