About

The Flow Cytometry and Confocal Microscopy Core works with research labs within Mayo Clinic in Arizona and the local community to ensure access to needed research equipment. In addition to Mayo Clinic labs, the core works with Translational Genomics Research Institute (TGen), Arizona State University and the University of Arizona.

The core is located in the Samuel C. Johnson Medical Research Building at Mayo Clinic's campus in Scottsdale.

Equipment

Beckman Coulter Cyan ADP Analyzer flow cytometer

  • Summit Software,
  • Equipped with three lasers: 405 nanometers (nm), 488 nm and 635 nm
  • Nine colors

BD Biosciences LSRFortessa cell analyzer

  • FACSDiva Software
  • Equipped with five lasers: 355 nm, 405 nm, 488 nm, 560 nm and 635 nm
  • 16 colors

BD Biosciences FACSAria III SORP cell sorter

  • FACSDiva Software
  • Equipped with five lasers: 355 nm, 405 nm, 488 nm, 560 nm and 635 nm
  • 16 colors
  • Contained within a Baker BioProtect safety cabinet
  • Automatic cell deposition unit to sort into 96 well plates
  • Chilled tube holder

Zeiss LSM 510 confocal microscope

  • LSM 510 Image Browser software
  • Laser excitations: 405 nm, 458 nm, 488 nm, 514 nm, 543nm and 633 nm
  • Heat and carbon dioxide for time series experiments
  • Eppendorf microinjection apparatus
  • Three channels (colors) at one time
  • Channel 1 has the following filter sets: LP 420, LP 475, LP 505, LP 530, LP 560, LP 650 and BP 565-615
  • Channel 2 has the following filter sets: BP 420-480, BP 390-465, BP 435-485, BP 480-520, BP 500-530, BP 500-550 and LP 505
  • Channel 3 has the following filter sets: LP 560, BP 500-550, BP 535-590 and BP 565-615

Scheduling

Online scheduling is available for Mayo staff. External users should contact Carole M. Viso at viso.carole@mayo.edu to schedule time on the instruments.