C. Elegans PKD-targeted Services
Since it is extremely difficult to study cilia biology and the connections among cilia formation, sensory function and disease in mammalian model organisms, alternative experimental systems such as C. elegans are necessary.
C. elegans — with its evolutionarily conserved PKD genes, polycystin ciliary localization and polycystin sensory function — is a powerful model for characterizing the physiological roles of PKD genes in their native cellular environment.
Services and rodent models provided by the Translational Polycystic Kidney Disease Center's Model Systems Core at Mayo are available to PKD researchers at Mayo Clinic and other institutions.
The Model Systems Core offers five C. elegans PKD-targeted services:
- Small-molecular screening for cystic phenotype effects, such as mating behavior and dye filling
- Access to worm mutants involved in the polycystin signaling pathway
- Analysis of genetic, molecular and cellular interactions of evolutionarily conserved candidate genes with polycystins and other components involved in polycystin signaling
- Screens for deletion mutants of worm homologs of interesting mammalian genes
- Training on C. elegans-related technologies (cilia structure and function, worm genetics, and whole-genome mutagenesis and high-throughput RNAi screenings)
We have dozens of PKD-related mutants as well as GFP-tagged transgenic animals expressing the polycystins and other ciliary markers frozen in the core. For a detailed list, contact the core.
For more information about C. elegans services offered through the Model Systems Core, contact the core.